PDBsum entry 4zfp

Hydrolase

PDB id

4zfp

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Contents

			Protein chain

					129 a.a.

			Ligands

					EDO


					NO3 ×6

			Metals

					_AU ×3

			Waters ×127

PDB id:

4zfp

Links

Name:

Hydrolase

Title:

A new crystal structure for the adduct formed in the reaction between ausac2, a cytotoxic homoleptic gold(i) compound with the saccharinate ligand, and the model protein hen egg white lysozyme

Structure:

LysozymE C. Chain: a. Synonym: 1,4-beta-n-acetylmuramidasE C,allergen gal d iv. Ec: 3.2.1.17

Source:

Gallus gallus. Chicken. Organism_taxid: 9031

Resolution:

1.96Å

R-factor:

0.173

R-free:

0.235

Authors:

A.Merlino

Key ref:

G.Ferraro et al. (2015). Structural evidences for a secondary gold binding site in the hydrophobic box of lysozyme. Biometals, 28, 745-754. PubMed id: 26054833 DOI: 10.1007/s10534-015-9863-7

Date:

21-Apr-15

Release date:

24-Jun-15

PROCHECK

	Headers

	References

Protein chain

P00698 (LYSC_CHICK) - Lysozyme C from Gallus gallus

Seq: Struc:					147 a.a. 129 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.3.2.1.17 - lysozyme.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.

DOI no: 10.1007/s10534-015-9863-7	Biometals 28:745-754 (2015)
PubMed id: 26054833

Structural evidences for a secondary gold binding site in the hydrophobic box of lysozyme.
G.Ferraro, L.Massai, L.Messori, M.A.Cinellu, A.Merlino.

ABSTRACT

A new crystal structure is reported here for the adduct formed in the reaction between NH4 [Au(Sac)2], AuSac2, a cytotoxic homoleptic gold(I) complex with the saccharinate ligand, and the model protein hen egg white lysozyme. To produce this adduct, AuSac2 breaks down and releases both saccharinate ligands. The resulting Au(I) ions bind the protein to ND1 and NE2 atoms of His15 but also to SD atom of the zero-solvent accessible Met105 side chain, which is located in the protein hydrophobic box. The unexpected existence of this secondary gold(I) binding site is confirmed by spectroscopic and spectrometric measurements in solution.