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PDBsum entry 4zfi
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PDB id:
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Ligase
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Title:
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Structure of mdm2 with low molecular weight inhibitor
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Structure:
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E3 ubiquitin-protein ligase mdm2. Chain: a, b, c, d. Fragment: unp residues 18-113. Synonym: double minute 2 protein,hdm2,oncoprotein mdm2,p53-binding protein mdm2. Engineered: yes. Other_details: p53 binding domain of mdm2
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: mdm2. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
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Resolution:
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2.00Å
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R-factor:
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0.200
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R-free:
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0.243
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Authors:
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K.M.Zak,A.Twarda-Clapa,E.M.Wrona,P.Grudnik,G.Dubin,T.A.Holak
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Key ref:
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E.Surmiak
et al.
(2016).
A Unique Mdm2-Binding Mode of the 3-Pyrrolin-2-one- and 2-Furanone-Based Antagonists of the p53-Mdm2 Interaction.
Acs Chem Biol,
11,
3310-3318.
PubMed id:
DOI:
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Date:
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21-Apr-15
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Release date:
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19-Oct-16
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PROCHECK
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Headers
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References
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Q00987
(MDM2_HUMAN) -
E3 ubiquitin-protein ligase Mdm2 from Homo sapiens
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Seq:
Struc:
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491 a.a.
95 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Enzyme class:
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E.C.2.3.2.27
- RING-type E3 ubiquitin transferase.
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Reaction:
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S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6- ubiquitinyl-[acceptor protein]-L-lysine
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DOI no:
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Acs Chem Biol
11:3310-3318
(2016)
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PubMed id:
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A Unique Mdm2-Binding Mode of the 3-Pyrrolin-2-one- and 2-Furanone-Based Antagonists of the p53-Mdm2 Interaction.
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E.Surmiak,
A.Twarda-Clapa,
K.M.Zak,
B.Musielak,
M.D.Tomala,
K.Kubica,
P.Grudnik,
M.Madej,
M.Jablonski,
J.Potempa,
J.Kalinowska-Tluscik,
A.Dömling,
G.Dubin,
T.A.Holak.
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ABSTRACT
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