PDBsum entry 4zdj

Ligase

PDB id

4zdj

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Contents

			Protein chain

					535 a.a.

			Ligands

					UTP ×2


					GOL

			Metals

					_MG ×2

			Waters ×226

PDB id:

4zdj

Links

Name:

Ligase

Title:

Crystal structure of the m. Tuberculosis ctp synthase pyrg in complex with two utp molecules

Structure:

Ctp synthase. Chain: a. Synonym: ctp synthetase,utp--ammonia ligase. Engineered: yes

Source:

Mycobacterium tuberculosis (strain atcc 25618 / h37rv). Organism_taxid: 83332. Gene: pyrg, rv1699, mtci125.21. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.99Å

R-factor:

0.168

R-free:

0.189

Authors:

M.Bellinzoni,N.Barilone,P.M.Alzari

Key ref:

G.Mori et al. (2015). Thiophenecarboxamide Derivatives Activated by EthA Kill Mycobacterium tuberculosis by Inhibiting the CTP Synthetase PyrG. Chem Biol, 22, 917-927. PubMed id: 26097035 DOI: 10.1016/j.chembiol.2015.05.016

Date:

17-Apr-15

Release date:

01-Jul-15

PROCHECK

	Headers

	References

Protein chain

P9WHK7 (PYRG_MYCTU) - CTP synthase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: Struc:

Seq: Struc:					586 a.a. 535 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.6.3.4.2 - Ctp synthase (glutamine hydrolyzing).

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H⁺

UTP	+	L-glutamine	+	ATP	+	H2O Bound ligand (Het Group name = UTP) corresponds exactly	=	CTP	+	L-glutamate	+	ADP	+	phosphate	+	2 × H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.chembiol.2015.05.016	Chem Biol 22:917-927 (2015)
PubMed id: 26097035

Thiophenecarboxamide Derivatives Activated by EthA Kill Mycobacterium tuberculosis by Inhibiting the CTP Synthetase PyrG.
G.Mori, L.R.Chiarelli, M.Esposito, V.Makarov, M.Bellinzoni, R.C.Hartkoorn, G.Degiacomi, F.Boldrin, S.Ekins, A.L.de Jesus Lopes Ribeiro, L.B.Marino, I.Centárová, Z.Svetlíková, J.Blaško, E.Kazakova, A.Lepioshkin, N.Barilone, G.Zanoni, A.Porta, M.Fondi, R.Fani, A.R.Baulard, K.Mikušová, P.M.Alzari, R.Manganelli, L.P.de Carvalho, G.Riccardi, S.T.Cole, M.R.Pasca.

ABSTRACT

No abstract given.