PDBsum entry 4zdi

Ligase

PDB id: 4zdi

Contents

Protein chains

(+ 2 more) 531 a.a.

Metals

_CA ×8

PDB id:

4zdi

Name:

Ligase

Title:

Crystal structure of the m. Tuberculosis ctp synthase pyrg (apo form)

Structure:

Ctp synthase. Chain: a, b, c, d, e, f, g, h. Synonym: ctp synthetase,utp--ammonia ligase. Engineered: yes

Source:

Mycobacterium tuberculosis (strain atcc 25618 / h37rv). Organism_taxid: 83332. Gene: pyrg, rv1699, mtci125.21. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

3.52Å R-factor: 0.213 R-free: 0.221

Authors:

M.Bellinzoni,N.Barilone,P.M.Alzari

Key ref:

G.Mori et al. (2015). Thiophenecarboxamide Derivatives Activated by EthA Kill Mycobacterium tuberculosis by Inhibiting the CTP Synthetase PyrG. Chem Biol, 22, 917-927. PubMed id: 26097035 DOI: 10.1016/j.chembiol.2015.05.016

Date:

17-Apr-15 Release date: 01-Jul-15

Protein chains

P9WHK7  (PYRG_MYCTU) -  CTP synthase from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 586 a.a.

Struc: 531 a.a.

Enzyme reactions

• Enzyme class: E.C.6.3.4.2 - Ctp synthase (glutamine hydrolyzing).
• Reaction: UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.chembiol.2015.05.016

Chem Biol 22:917-927 (2015)

PubMed id: 26097035

Thiophenecarboxamide Derivatives Activated by EthA Kill Mycobacterium tuberculosis by Inhibiting the CTP Synthetase PyrG.

G.Mori, L.R.Chiarelli, M.Esposito, V.Makarov, M.Bellinzoni, R.C.Hartkoorn, G.Degiacomi, F.Boldrin, S.Ekins, A.L.de Jesus Lopes Ribeiro, L.B.Marino, I.Centárová, Z.Svetlíková, J.Blaško, E.Kazakova, A.Lepioshkin, N.Barilone, G.Zanoni, A.Porta, M.Fondi, R.Fani, A.R.Baulard, K.Mikušová, P.M.Alzari, R.Manganelli, L.P.de Carvalho, G.Riccardi, S.T.Cole, M.R.Pasca.

ABSTRACT

No abstract given.