PDBsum entry 4z8k

Photosynthesis

PDB id: 4z8k

Contents

Protein chains

162 a.a.

172 a.a.

Ligands

CYC ×3

Waters ×98

PDB id:

4z8k

Name:

Photosynthesis

Title:

Phycocyanin structure from t. Elongatus at 2.5-a from xfel using a viscous delivery medium for serial femtosecond crystallography

Structure:

C-phycocyanin alpha chain. Chain: a. C-phycocyanin beta chain. Chain: b

Source:

Thermosynechococcus elongatus (strain bp-1). Organism_taxid: 197221. Strain: bp-1. Strain: bp-1

Resolution:

2.50Å R-factor: 0.191 R-free: 0.255

Authors:

R.Fromme,S.Basu,P.Fromme

Key ref:

C.E.Conrad et al. (2015). A novel inert crystal delivery medium for serial femtosecond crystallography. Iucrj, 2, 421-430. PubMed id: 26177184 DOI: 10.1107/S2052252515009811

Date:

09-Apr-15 Release date: 12-Aug-15

Protein chain

P50032  (PHCA_THEEB) -  C-phycocyanin alpha subunit from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 162 a.a.

Struc: 162 a.a.*

Protein chain

P50033  (PHCB_THEEB) -  C-phycocyanin beta subunit from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)

Seq: 172 a.a.

Struc: 172 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DOI no: 10.1107/S2052252515009811

Iucrj 2:421-430 (2015)

PubMed id: 26177184

A novel inert crystal delivery medium for serial femtosecond crystallography.

C.E.Conrad, S.Basu, D.James, D.Wang, A.Schaffer, S.Roy-Chowdhury, N.A.Zatsepin, A.Aquila, J.Coe, C.Gati, M.S.Hunter, J.E.Koglin, C.Kupitz, G.Nelson, G.Subramanian, T.A.White, Y.Zhao, J.Zook, S.Boutet, V.Cherezov, J.C.Spence, R.Fromme, U.Weierstall, P.Fromme.

ABSTRACT

Serial femtosecond crystallography (SFX) has opened a new era in crystallo-graphy by permitting nearly damage-free, room-temperature structure determination of challenging proteins such as membrane proteins. In SFX, femtosecond X-ray free-electron laser pulses produce diffraction snapshots from nanocrystals and microcrystals delivered in a liquid jet, which leads to high protein consumption. A slow-moving stream of agarose has been developed as a new crystal delivery medium for SFX. It has low background scattering, is compatible with both soluble and membrane proteins, and can deliver the protein crystals at a wide range of temperatures down to 4°C. Using this crystal-laden agarose stream, the structure of a multi-subunit complex, phycocyanin, was solved to 2.5 Å resolution using 300 µg of microcrystals embedded into the agarose medium post-crystallization. The agarose delivery method reduces protein consumption by at least 100-fold and has the potential to be used for a diverse population of proteins, including membrane protein complexes.