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PDBsum entry 4z6a
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protein ligands metals Protein-protein interface(s) links
Hydrolase PDB id
4z6a

 

 

 

 

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Contents
Protein chains
94 a.a.
249 a.a.
201 a.a.
Ligands
BGC
FUC
0Z6
CIT
Metals
_CA
Waters ×230
PDB id:
4z6a
Name: Hydrolase
Title: Crystal structure of a fviia-trypsin chimera (yt) in complex with soluble tissue factor
Structure: Coagulation factor vii. Chain: l. Fragment: unp residues 108-203. Synonym: proconvertin,serum prothrombin conversion accelerator,spca. Engineered: yes. Coagulation factor vii. Chain: h. Fragment: unp residues 213-465. Synonym: proconvertin,serum prothrombin conversion accelerator,spca.
Source: Homo sapiens. Human. Organism_taxid: 9606. Organ: kidney. Tissue: blood. Gene: f7. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: choebnalt85.
Resolution:
2.25Å     R-factor:   0.191     R-free:   0.241
Authors: A.B.Sorensen,L.A.Svensson,P.S.Gandhi
Key ref: A.B.Sorensen et al. (2016). Molecular Basis of Enhanced Activity in Factor VIIa-Trypsin Variants Conveys Insights into Tissue Factor-mediated Allosteric Regulation of Factor VIIa Activity. J Biol Chem, 291, 4671-4683. PubMed id: 26694616 DOI: 10.1074/jbc.M115.698613
Date:
04-Apr-15     Release date:   30-Dec-15    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P08709  (FA7_HUMAN) -  Coagulation factor VII from Homo sapiens
Seq:
Struc: 		466 a.a.
94 a.a.
Protein chain
Pfam   ArchSchema ?
P08709  (FA7_HUMAN) -  Coagulation factor VII from Homo sapiens
Seq:
Struc: 		466 a.a.
249 a.a.*
Protein chain
Pfam   ArchSchema ?
P13726  (TF_HUMAN) -  Tissue factor from Homo sapiens
Seq:
Struc: 		295 a.a.
201 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 6 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains L, H: E.C.3.4.21.21  - coagulation factor VIIa.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Hydrolyzes one Arg-|-Ile bond in factor X to form factor Xa.

 

 
DOI no: 10.1074/jbc.M115.698613 J Biol Chem 291:4671-4683 (2016)
PubMed id: 26694616  
 
 
Molecular Basis of Enhanced Activity in Factor VIIa-Trypsin Variants Conveys Insights into Tissue Factor-mediated Allosteric Regulation of Factor VIIa Activity.
A.B.Sorensen, J.J.Madsen, L.A.Svensson, A.A.Pedersen, H.Østergaard, M.T.Overgaard, O.H.Olsen, P.S.Gandhi.
 
  ABSTRACT  
 
No abstract given.

 

 

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