PDBsum entry 4z4q

Isomerase

PDB id: 4z4q

Contents

Protein chains

690 a.a.

DNA/RNA

Ligands

PDQ ×2

Metals

_MG ×4

Waters ×8

PDB id:

4z4q

Name:

Isomerase

Title:

Quinazolinedione(pd 0305970)-DNA cleavage complex of topoisomerase iv from s. Pneumoniae

Structure:

DNA topoisomerase 4 subunit b,DNA topoisomerase 4 subunit a. Chain: a, b. Synonym: topoisomerase iv subunit b,topoisomerase iv subunit a. Engineered: yes. V-site DNA. Chain: e. Engineered: yes. V-site DNA.

Source:

Streptococcus pneumoniae serotype 4 (strain atcc baa-334 / tigr4). Organism_taxid: 170187. Strain: atcc baa-334 / tigr4. Gene: pare, sp_0852, parc, sp_0855. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Pbr322 based ig-lambda expression vector.

Resolution:

3.04Å R-factor: 0.164 R-free: 0.205

Authors:

I.Laponogov,D.A.Veselkov,X.-S.Pan,J.Selvarajah,I.M.-T.Crevel, L.M.Fisher,M.R.Sanderson

Key ref:

I.Laponogov et al. Structural studies of the drug-Stabilized cleavage co of topoisomerase IV and gyrase from streptococcus pne. To be published, .

Date:

02-Apr-15 Release date: 14-Sep-16

Protein chains

P72525  (PARC_STRPN) -  DNA topoisomerase 4 subunit A from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)

Seq: 823 a.a.

Struc: 690 a.a.*

Protein chains

Q59961  (PARE_STRPN) -  DNA topoisomerase 4 subunit B from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)

Seq: 647 a.a.

Struc: 690 a.a.*

* PDB and UniProt seqs differ at 11 residue positions (black crosses)

DNA/RNA chains

G-T-A-A-T-A-C 7 bases

A-A-C-C-G-T-A-T-T-A-C 11 bases

T-G-T-G-G-A-T 7 bases

G-G-T-T-A-T-C-C-A-C-A 11 bases

Enzyme reactions

• Enzyme class: E.C.5.6.2.2 - Dna topoisomerase (ATP-hydrolyzing).