PDBsum entry 4z3b

Iron storage

PDB id

4z3b

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Contents

			Protein chain

					174 a.a.

			Ligands

					GOL


					SO4

			Metals

					_CD ×3


					_MN ×2

			Waters ×176

PDB id:

4z3b

Links

Name:

Iron storage

Title:

Crystal structure of mnco/apo-r52cfr

Structure:

Ferritin light chain. Chain: a. Fragment: unp residues 2-175. Synonym: ferritin l subunit. Engineered: yes

Source:

Equus caballus. Horse. Organism_taxid: 9796. Gene: ftl. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.42Å

R-factor:

0.167

R-free:

0.177

Authors:

K.Fujita,Y.Tanaka,S.Abe,T.Ueno

Key ref:

K.Fujita et al. (2016). A Photoactive Carbon-Monoxide-Releasing Protein Cage for Dose-Regulated Delivery in Living Cells. Angew Chem Int Ed Engl, 55, 1056-1060. PubMed id: 26332099 DOI: 10.1002/anie.201506738

Date:

31-Mar-15

Release date:

21-Oct-15

PROCHECK

	Headers

	References

Protein chain

P02791 (FRIL_HORSE) - Ferritin light chain from Equus caballus

Seq: Struc:					175 a.a. 174 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1002/anie.201506738	Angew Chem Int Ed Engl 55:1056-1060 (2016)
PubMed id: 26332099

A Photoactive Carbon-Monoxide-Releasing Protein Cage for Dose-Regulated Delivery in Living Cells.
K.Fujita, Y.Tanaka, S.Abe, T.Ueno.

ABSTRACT

Protein cages can serve as bioinorganic molecular templates for functionalizing metal compounds to regulate cellular signaling. We succeeded in developing a photoactive CO-releasing system by constructing a composite of ferritin (Fr) containing manganese-carbonyl complexes. When Arg52 adjacent to Cys48 of Fr is replaced with Cys, the Fr mutant stabilizes the retention of 48 Mn-carbonyl moieties, which can release the CO ligands under light irradiation, although wild-type Fr retains very few Mn moieties. The amount of released CO is regulated by the extent of irradiation. This could reveal an optimized dose for cooperatively activating the nuclear factor κB (NF-κB) in mammalian cells and the tumor necrosis factor α (TNF-α). These results suggest that construction of a CO-releasing protein cage will advance of research in CO biology.