PDBsum entry 4z2t

Oxidoreductase

PDB id: 4z2t

Contents

Protein chains

559 a.a.

Ligands

FAD ×2

Waters ×455

PDB id:

4z2t

Name:

Oxidoreductase

Title:

Crystal structure of 2-hydroxybiphenyl 3-monooxygenase w225y from pseudomonas azelaica

Structure:

2-hydroxybiphenyl-3-monooxygenase. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Pseudomonas nitroreducens hbp1. Organism_taxid: 1437882. Gene: hbpa. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.45Å R-factor: 0.219 R-free: 0.236

Authors:

M.Kanteev,A.Bregman-Cohen,A.Fishman

Key ref:

M.Kanteev et al. (2015). A crystal structure of 2-hydroxybiphenyl 3-monooxygenase with bound substrate provides insights into the enzymatic mechanism. Biochim Biophys Acta, 1854, 1906-1913. PubMed id: 26275805 DOI: 10.1016/j.bbapap.2015.08.002

Date:

30-Mar-15 Release date: 19-Aug-15

Protein chains

O06647  (O06647_PSENT) -  2-hydroxybiphenyl-3-monooxygenase from Pseudomonas nitroreducens

Seq: 586 a.a.

Struc: 559 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.14.13.44 - 2-hydroxybiphenyl 3-monooxygenase.
• Reaction: biphenyl-2-ol + NADH + O2 + H⁺ = biphenyl-2,3-diol + NAD⁺ + H2O

biphenyl-2-ol + NADH + O2 (Bound ligand (Het Group name = FAD) matches with 76.36% similarity) + H(+) = biphenyl-2,3-diol + NAD(+) + H2O

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.bbapap.2015.08.002

Biochim Biophys Acta 1854:1906-1913 (2015)

PubMed id: 26275805

A crystal structure of 2-hydroxybiphenyl 3-monooxygenase with bound substrate provides insights into the enzymatic mechanism.

M.Kanteev, A.Bregman-Cohen, B.Deri, A.Shahar, N.Adir, A.Fishman.

ABSTRACT

No abstract given.