PDBsum entry 4z2a

Hydrolase

PDB id: 4z2a

Contents

Protein chain

465 a.a.

Ligands

PO4 ×2

EDO

Metals

_CA ×4

Waters ×408

PDB id:

4z2a

Name:

Hydrolase

Title:

Crystal structure of unglycosylated apo human furin @1.89a

Structure:

Furin. Chain: a. Fragment: unp residues 110-74. Synonym: dibasic-processing enzyme,paired basic amino acid residue- cleaving enzyme,pace. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: furin, fur, pace, pcsk3. Expressed in: cricetulus griseus. Expression_system_taxid: 10029

Resolution:

1.89Å R-factor: 0.149 R-free: 0.183

Authors:

R.T.Gampe,K.Pearce,R.Reid

Key ref:

K.Pearce A bacmam method for producing high levels of active h furin protein for biochemical studies and crystallogr. To be published, .

Date:

29-Mar-15 Release date: 04-May-16

Protein chain

P09958  (FURIN_HUMAN) -  Furin from Homo sapiens

Seq: 794 a.a.

Struc: 465 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.21.75 - furin.
• Reaction: Release of mature proteins from their proproteins by cleavage of Arg- Xaa-Yaa-Arg-|-Zaa bonds, where Xaa can be any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and von Willebrand factor from their respective precursors.