PDBsum entry 4z1x

Hydrolase/DNA

PDB id: 4z1x

Contents

Protein chains

291 a.a.

DNA/RNA

Metals

_CA ×4

Waters ×129

PDB id:

4z1x

Name:

Hydrolase/DNA

Title:

Crystal structure of laglidadg homing endonuclease i-gzeii in complex with DNA target

Structure:

DNA (27-mer). Chain: c, e. Engineered: yes. DNA (27-mer). Chain: d, f. Engineered: yes. Laglidadg endonuclease. Chain: a, b. Synonym: i-gzeii.

Source:

Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Fusarium graminearum ph-1. Wheat head blight fungus. Organism_taxid: 229533. Gene: nad2. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.80Å R-factor: 0.176 R-free: 0.240

Authors:

B.L.Stoddard,A.R.Lambert,N.Kulshina

Key ref:

B.L.Stoddard et al. Crystal structure of laglidadg homing endonuclease i- In complex with DNA target. To be published, .

Date:

27-Mar-15 Release date: 22-Apr-15

Protein chains

A5J036  (A5J036_GIBZE) -  LAGLIDADG endonuclease from Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)

Seq: 348 a.a.

Struc: 291 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

DNA/RNA chains

G-G-A-T-G-G-G-T-A-C-C-A-T-A-T-T-G-G-T-A-C-A-A-A-G-G-G 27 bases

C-C-T-T-T-G-T-A-C-C-A-A-T-A-T-G-G-T-A-C-C-C-A-T-C-C 26 bases

G-G-A-T-G-G-G-T-A-C-C-A-T-A-T-T-G-G-T-A-C-A-A-A-G-G-G 27 bases

C-C-T-T-T-G-T-A-C-C-A-A-T-A-T-G-G-T-A-C-C-C-A-T-C-C 26 bases