PDBsum entry 4yif

DNA binding protein

PDB id: 4yif

Contents

Protein chains

140 a.a.

132 a.a.

Waters ×529

PDB id:

4yif

Name:

DNA binding protein

Title:

Crystal structure of rv0880

Structure:

Marr family protein rv0880. Chain: a, b, c, d, e, f. Fragment: unp residues 2-143. Engineered: yes

Source:

Mycobacterium tuberculosis. Organism_taxid: 83332. Strain: atcc 25618 / h37rv. Gene: rv0880, mtcy31.08. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.00Å R-factor: 0.214 R-free: 0.249

Authors:

Y.R.Gao,N.Feng,D.F.Li,L.J.Bi

Key ref:

Y.R.Gao et al. (2015). Structure of the MarR family protein Rv0880 from Mycobacterium tuberculosis. Acta Crystallogr F Struct Biol Commun, 71, 741-745. PubMed id: 26057805 DOI: 10.1107/S2053230X15007281

Date:

02-Mar-15 Release date: 10-Jun-15

Protein chains

P9WMF1  (Y880_MYCTU) -  HTH-type transcriptional regulator Rv0880 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 143 a.a.

Struc: 140 a.a.

Protein chains

P9WMF1  (Y880_MYCTU) -  HTH-type transcriptional regulator Rv0880 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 143 a.a.

Struc: 132 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F: E.C.?

DOI no: 10.1107/S2053230X15007281

Acta Crystallogr F Struct Biol Commun 71:741-745 (2015)

PubMed id: 26057805

Structure of the MarR family protein Rv0880 from Mycobacterium tuberculosis.

Y.R.Gao, N.Feng, T.Chen, d.e. .F.Li, L.J.Bi.

ABSTRACT

Rv0880 from the pathogen Mycobacterium tuberculosis is classified as a MarR family protein in the Pfam database. It consists of 143 amino acids and has an isoelectric point of 10.9. Crystals of Rv0880 belonged to space group P1, with unit-cell parameters a = 54.97, b = 69.60, c = 70.32 Å, α = 103.71, β = 111.06, γ = 105.83°. The structure of the MarR family transcription regulator Rv0880 was solved at a resolution of 2.0 Å with an R(cryst) and R(free) of 21.2 and 24.9%, respectively. The dimeric structure resembles that of other MarR proteins, with each subunit comprising a winged helix-turn-helix domain connected to an α-helical dimerization domain.