PDBsum entry 4xo2

Protein binding

PDB id: 4xo2

Contents

Protein chains

60 a.a.

Waters ×46

PDB id:

4xo2

Name:

Protein binding

Title:

Crystal structure of gnsa from e.Coli

Structure:

Protein gnsa. Chain: a, b. Engineered: yes

Source:

Escherichia coli (strain k12). Organism_taxid: 83333. Strain: k12. Gene: gnsa, yccl, b4517, jw0976. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.95Å R-factor: 0.217 R-free: 0.250

Authors:

L.H.Zhan,Z.Q.Gao,Y.Wei,Y.H.Dong

Key ref:

Y.Wei et al. (2015). Crystal structure of GnsA from Escherichia coli. Biochem Biophys Res Commun, 462, 1-7. PubMed id: 25839658 DOI: 10.1016/j.bbrc.2015.03.133

Date:

16-Jan-15 Release date: 17-Jun-15

Protein chains

P0AC92  (GNSA_ECOLI) -  Protein GnsA from Escherichia coli (strain K12)

Seq: 57 a.a.

Struc: 60 a.a.

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1016/j.bbrc.2015.03.133

Biochem Biophys Res Commun 462:1-7 (2015)

PubMed id: 25839658

Crystal structure of GnsA from Escherichia coli.

Y.Wei, L.Zhan, Z.Gao, G.G.Privé, Y.Dong.

ABSTRACT

Escherichia Coli GnsA is a regulator of phosphatidylethanolamine synthesis and functions as a suppressor of both a secG null mutation and fabA6 mutations. GnsA may also be a toxin with the cognate antitoxin YmcE. Here we report the crystal structure of GnsA to 1.8 Å. GnsA forms a V shaped hairpin structure that is tightly associated into a homodimer. Our comprehensive structural study suggests that GnsA is structurally similar to an outer membrane protein, suggesting a function of protein binding.