PDBsum entry 4xnh

Transferase

PDB id: 4xnh

Contents

Protein chains

786 a.a.

197 a.a.

159 a.a.

Ligands

SER-TYR-SER-MET-GLU-HIS

IHP

ACO

CMC

Waters ×672

PDB id:

4xnh

Name:

Transferase

Title:

Crystal structure of yeast n-terminal acetyltransferase nate (ip6) in complex with a bisubstrate

Structure:

N-terminal acetyltransferase a complex subunit nat1. Chain: a. Synonym: nata complex subunit nat1,amino-terminal,alpha-amino, acetyltransferase 1. Engineered: yes. N-terminal acetyltransferase a complex catalytic subunit ard1. Chain: b. Synonym: nata complex subunit ard1,arrest-defective protein 1.

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Homo sapiens. Human. Organism_taxid: 9606

Resolution:

2.10Å R-factor: 0.171 R-free: 0.228

Authors:

J.Dong,S.Wang,J.D.York

Key ref:

J.Dong et al. Crystal structure of yeast n-Terminal acetyltransfera (ip6) in complex with a bisubstrate. To be published, .

Date:

15-Jan-15 Release date: 20-Jul-16

Protein chain

P12945  (NAT1_YEAST) -  N-terminal acetyltransferase A complex subunit NAT1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 854 a.a.

Struc: 786 a.a.

Protein chain

P07347  (ARD1_YEAST) -  N-terminal acetyltransferase A complex catalytic subunit ARD1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 238 a.a.

Struc: 197 a.a.

Protein chain

Q08689  (NAT5_YEAST) -  N-alpha-acetyltransferase NAT5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 176 a.a.

Struc: 159 a.a.

Enzyme reactions

• Enzyme class 2: Chain A: E.C.?
• Enzyme class 3: Chain B: E.C.2.3.1.255 - N-terminal amino-acid N(alpha)-acetyltransferase NatA.
• Reaction:
  1. N-terminal glycyl-[protein] + acetyl-CoA = N-terminal N(alpha)- acetylglycyl-[protein] + CoA + H⁺
  2. N-terminal L-alanyl-[protein] + acetyl-CoA = N-terminal N(alpha)- acetyl-L-alanyl-[protein] + CoA + H⁺
  3. N-terminal L-seryl-[protein] + acetyl-CoA = N-terminal N(alpha)- acetyl-L-seryl-[protein] + CoA + H⁺
  4. N-terminal L-valyl-[protein] + acetyl-CoA = N-terminal N(alpha)- acetyl-L-valyl-[protein] + CoA + H⁺
  5. N-terminal L-cysteinyl-[protein] + acetyl-CoA = N-terminal N(alpha)- acetyl-L-cysteinyl-[protein] + CoA + H⁺
  6. N-terminal L-threonyl-[protein] + acetyl-CoA = N-terminal N(alpha)- acetyl-L-threonyl-[protein] + CoA + H⁺

N-terminal glycyl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terminal N(alpha)- acetylglycyl-[protein] + CoA + H(+)

N-terminal L-alanyl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terminal N(alpha)- acetyl-L-alanyl-[protein] + CoA + H(+)

N-terminal L-seryl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terminal N(alpha)- acetyl-L-seryl-[protein] + CoA + H(+)

N-terminal L-valyl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terminal N(alpha)- acetyl-L-valyl-[protein] + CoA + H(+)

N-terminal L-cysteinyl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terminal N(alpha)- acetyl-L-cysteinyl-[protein] + CoA + H(+)

N-terminal L-threonyl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terminal N(alpha)- acetyl-L-threonyl-[protein] + CoA + H(+)

• Enzyme class 4: Chain C: E.C.2.3.1.258 - N-terminal methionine N(alpha)-acetyltransferase NatE.
• Reaction:
  1. N-terminal L-methionyl-L-alanyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-alanyl-[protein] + CoA + H⁺
  2. N-terminal L-methionyl-L-seryl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-seryl-[protein] + CoA + H⁺
  3. N-terminal L-methionyl-L-valyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-valyl-[protein] + CoA + H⁺
  4. N-terminal L-methionyl-L-threonyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-threonyl-[protein] + CoA + H⁺
  5. N-terminal L-methionyl-L-lysyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-lysyl-[protein] + CoA + H⁺
  6. N-terminal L-methionyl-L-leucyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-leucyl-[protein] + CoA + H⁺
  7. N-terminal L-methionyl-L-phenylalanyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-phenylalanyl-[protein] + CoA + H⁺
  8. N-terminal L-methionyl-L-tyrosyl-[protein] + acetyl-CoA = N-terCC -!- N-terminal-acetylases (NATs) catalyze the covalent attachment of an

N-terminal L-methionyl-L-alanyl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-alanyl-[protein] + CoA + H(+)

N-terminal L-methionyl-L-seryl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-seryl-[protein] + CoA + H(+)

N-terminal L-methionyl-L-valyl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-valyl-[protein] + CoA + H(+)

N-terminal L-methionyl-L-threonyl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-threonyl-[protein] + CoA + H(+)

N-terminal L-methionyl-L-lysyl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-lysyl-[protein] + CoA + H(+)

N-terminal L-methionyl-L-leucyl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-leucyl-[protein] + CoA + H(+)

N-terminal L-methionyl-L-phenylalanyl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-phenylalanyl-[protein] + CoA + H(+)

N-terminal L-methionyl-L-tyrosyl-[protein] (Bound ligand (Het Group name = ACO) corresponds exactly) + acetyl-CoA = N-terCC -!- N-terminal-acetylases (NATs) catalyze the covalent attachment of an

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site