PDBsum entry 4xmi

Hydrolase

PDB id: 4xmi

Contents

Protein chain

658 a.a.

Ligands

DMS ×6

PO4

Waters ×734

PDB id:

4xmi

Name:

Hydrolase

Title:

Crystal structure of the k499g mutant of nanb sialidase from streptococcus pneumoniae

Structure:

Sialidase b. Chain: a. Fragment: unp residues 39-696. Synonym: neuraminidase b. Engineered: yes. Mutation: yes

Source:

Streptococcus pneumoniae serotype 4 (strain atcc baa-334 / tigr4). Organism_taxid: 170187. Gene: nanb, sp_1687. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.97Å R-factor: 0.167 R-free: 0.206

Authors:

P.Brear

Key ref:

G.W.Rogers et al. The hunt for serendipitous allosteric sites: discover novel allosteric inhibitor of the bacterial sialidase. To be published, .

Date:

14-Jan-15 Release date: 13-Jan-16

Protein chain

Q54727  (NANB_STRPN) -  Sialidase B from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)

Seq: 697 a.a.

Struc: 658 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.18 - exo-alpha-sialidase.
• Reaction: Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)-glycosidic linkages of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.