PDBsum entry 4xhl

Transferase/transferase inhibitor

PDB id: 4xhl

Contents

Protein chain

330 a.a.

Ligands

CKI

SO4 ×7

Waters ×7

PDB id:

4xhl

Name:

Transferase/transferase inhibitor

Title:

Structure of s. Cerevisiae hrr25 1-394 (k38r mutant)

Structure:

Casein kinase i homolog hrr25. Chain: a. Fragment: unp residues 1-394. Engineered: yes. Mutation: yes

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Gene: hrr25. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

3.01Å R-factor: 0.268 R-free: 0.285

Authors:

Q.Ye,K.D.Corbett

Key ref:

Q.Ye et al. (2016). Structure of the Saccharomyces cerevisiae Hrr25:Mam1 monopolin subcomplex reveals a novel kinase regulator. Embo J, 35, 2139-2151. PubMed id: 27491543 DOI: 10.15252/embj.201694082

Date:

05-Jan-15 Release date: 06-Jan-16

Protein chain

P29295  (HRR25_YEAST) -  Casein kinase I homolog HRR25 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 494 a.a.

Struc: 330 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.15252/embj.201694082

Embo J 35:2139-2151 (2016)

PubMed id: 27491543

Structure of the Saccharomyces cerevisiae Hrr25:Mam1 monopolin subcomplex reveals a novel kinase regulator.

Q.Ye, S.N.Ur, T.Y.Su, K.D.Corbett.

ABSTRACT

No abstract given.