PDBsum entry 4xgi

Oxidoreductase

PDB id: 4xgi

Contents

Protein chains

(+ 0 more) 416 a.a.

Ligands

NAD ×6

AKG ×6

EDO ×6

Waters ×1643

PDB id:

4xgi

Name:

Oxidoreductase

Title:

Crystal structure of glutamate dehydrogenase from burkholderia thailandensis

Structure:

Glutamate dehydrogenase. Chain: a, b, c, d, e, f. Fragment: butha.17991.A.A1. Engineered: yes

Source:

Burkholderia thailandensis (strain e264 / atcc 700388 / dsm 13276 / cip 106301). Organism_taxid: 271848. Strain: e264 / atcc 700388 / dsm 13276 / cip 106301. Atcc: 700388. Gene: bth_i1224, dr63_1118. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.00Å R-factor: 0.177 R-free: 0.222

Authors:

Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

J.Abendroth et al. Crystal structure of glutamate dehydrogenase from burkholderia thailandensis. To be published, .

Date:

30-Dec-14 Release date: 14-Jan-15

Protein chains

Q2SZ78  (Q2SZ78_BURTA) -  Glutamate dehydrogenase from Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CCUG 48851 / CIP 106301 / E264)

Seq: 434 a.a.

Struc: 416 a.a.

Enzyme reactions

• Enzyme class: E.C.1.4.1.4 - glutamate dehydrogenase (NADP(+)).
• Reaction: L-glutamate + NADP⁺ + H2O = 2-oxoglutarate + NH4⁺ + NADPH + H⁺

L-glutamate + NADP(+) + H2O (Bound ligand (Het Group name = NAD) matches with 91.67% similarity) = 2-oxoglutarate (Bound ligand (Het Group name = AKG) corresponds exactly) + NH4(+) + NADPH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site