PDBsum entry 4xdu

Transferase

PDB id

4xdu

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Contents

			Protein chain

					330 a.a.

			Ligands

					ADP


					EDO ×3


					ACT ×2

			Metals

					_MG ×2


					_NA ×2

			Waters ×255

PDB id:

4xdu

Links

Name:

Transferase

Title:

Crystal structure of treponema pallidum tp0796 flavin trafficking protein,a bifunctional fmn transferase/fad pyrophosphatase, n55y mutant, adp bound form

Structure:

Fad:protein fmn transferase. Chain: a. Synonym: flavin transferase. Engineered: yes. Mutation: yes

Source:

Treponema pallidum (strain nichols). Organism_taxid: 243276. Strain: nichols. Gene: apbe, tp_0796. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.35Å

R-factor:

0.169

R-free:

0.188

Authors:

D.R.Tomchick,C.A.Brautigam,R.K.Deka,M.V.Norgard

Key ref:

R.K.Deka et al. (2015). Evidence for Posttranslational Protein Flavinylation in the Syphilis Spirochete Treponema pallidum: Structural and Biochemical Insights from the Catalytic Core of a Periplasmic Flavin-Trafficking Protein. Mbio, 6, e00519. PubMed id: 25944861 DOI: 10.1128/mBio.00519-15

Date:

20-Dec-14

Release date:

14-Oct-15

PROCHECK

	Headers

	References

Protein chain

O83774 (APBE_TREPA) - FAD:protein FMN transferase from Treponema pallidum (strain Nichols)

Seq: Struc:					362 a.a. 330 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 2 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.2.7.1.180 - FAD:protein Fmn transferase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

L-threonyl-[protein] + FAD = FMN-L-threonyl-[protein] + AMP + H⁺

L-threonyl-[protein]	+	FAD	=	FMN-L-threonyl-[protein] Bound ligand (Het Group name = ADP) matches with 85.19% similarity	+	AMP	+	H(+)

Cofactor:

Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1128/mBio.00519-15	Mbio 6:e00519 (2015)
PubMed id: 25944861

Evidence for Posttranslational Protein Flavinylation in the Syphilis Spirochete Treponema pallidum: Structural and Biochemical Insights from the Catalytic Core of a Periplasmic Flavin-Trafficking Protein.
R.K.Deka, C.A.Brautigam, W.Z.Liu, D.R.Tomchick, M.V.Norgard.

ABSTRACT

No abstract given.