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PDBsum entry 4xau
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PDB id:
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Transferase
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Title:
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Crystal structure of ats13 from actinomadura melliaura
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Structure:
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Putative aminotransferase. Chain: a, b, c, d, e, f, g. Engineered: yes
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Source:
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Actinomadura melliaura. Organism_taxid: 360723. Gene: ats13. Expressed in: escherichia coli 'bl21-gold(de3)plyss ag'. Expression_system_taxid: 866768
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Resolution:
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3.00Å
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R-factor:
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0.188
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R-free:
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0.220
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Authors:
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F.Wang,S.Singh,W.Xu,J.S.Thorson,G.N.Phillips Jr.,Enzyme Discovery For Natural Product Biosynthesis (Natpro)
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Key ref:
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S.Singh
et al.
(2015).
Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis.
Proteins,
83,
1547-1554.
PubMed id:
DOI:
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Date:
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15-Dec-14
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Release date:
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24-Dec-14
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PROCHECK
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Headers
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References
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Q0H2X1
(Q0H2X1_9ACTN) -
Putative aminotransferase from Actinomadura melliaura
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Seq:
Struc:
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369 a.a.
372 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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DOI no:
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Proteins
83:1547-1554
(2015)
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PubMed id:
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Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis.
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S.Singh,
Y.Kim,
F.Wang,
L.Bigelow,
M.Endres,
M.K.Kharel,
G.Babnigg,
C.A.Bingman,
A.Joachimiak,
J.S.Thorson,
G.N.Phillips.
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ABSTRACT
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AT2433 from Actinomadura melliaura is an indolocarbazole antitumor antibiotic
structurally distinguished by its unique aminodideoxypentose-containing
disaccharide moiety. The corresponding sugar nucleotide-based biosynthetic
pathway for this unusual sugar derives from comparative genomics where AtmS13
has been suggested as the contributing sugar aminotransferase (SAT).
Determination of the AtmS13 X-ray structure at 1.50-Å resolution reveals it as
a member of the aspartate aminotransferase fold type I (AAT-I). Structural
comparisons of AtmS13 with homologous SATs that act upon similar substrates
implicate potential active site residues that contribute to distinctions in
sugar C5 (hexose vs. pentose) and/or sugar C2 (deoxy vs. hydroxyl) substrate
specificity. Proteins 2015; 83:1547-1554. © 2015 Wiley Periodicals, Inc.
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