PDBsum entry 4x0n

Hydrolase/hydrolase inhibitor

PDB id: 4x0n

Contents

Protein chains

496 a.a.

44 a.a.

Ligands

GOL

Metals

_CL

_CA

Waters ×219

PDB id:

4x0n

Name:

Hydrolase/hydrolase inhibitor

Title:

Porcine pancreatic alpha-amylase in complex with helianthamide, a novel proteinaceous inhibitor

Structure:

Pancreatic alpha-amylase. Chain: a. Fragment: unp residues 16-511. Synonym: pa,1,4-alpha-d-glucan glucanohydrolase. Helianthamide. Chain: b. Engineered: yes

Source:

Sus scrofa. Pig. Organism_taxid: 9823. Organ: pancreas. Synthetic: yes. Stichodactyla helianthus. Organism_taxid: 6123

Resolution:

2.60Å R-factor: 0.184 R-free: 0.226

Authors:

L.K.Williams,G.D.Brayer

Key ref:

C.Tysoe et al. Structural templating and guided refolding of the pot naturally occurring peptide helianthamide within the site of amylase, A diabetes and obesity therapeutic t. To be published, .

Date:

21-Nov-14 Release date: 25-Nov-15

Protein chain

P00690  (AMYP_PIG) -  Pancreatic alpha-amylase from Sus scrofa

Seq: 511 a.a.

Struc: 496 a.a.*

Protein chain

A0A0X1KGZ5  (IAA1_STIHL) -  Alpha-amylase inhibitor helianthamide from Stichodactyla helianthus

Seq: 44 a.a.

Struc: 44 a.a.

* PDB and UniProt seqs differ at 10 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.2.1.1 - alpha-amylase.
• Reaction: Endohydrolysis of 1,4-alpha-glucosidic linkages in oligosaccharides and polysaccharides.