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PDBsum entry 4wzc
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Oxidoreductase
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PDB id
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4wzc
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PDB id:
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Oxidoreductase
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Title:
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Understanding extradiol dioxygenase mechanism in NAD+ biosynthesis by viewing catalytic intermediates - 2,3-cis-4,5-trans acms bound to i142a mutant hao
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Structure:
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3-hydroxyanthranilate 3,4-dioxygenase. Chain: a. Synonym: 3-hydroxyanthranilate oxygenase,3-hao,3-hydroxyanthranilic acid dioxygenase,had. Engineered: yes. Mutation: yes
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Source:
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Ralstonia metallidurans. Organism_taxid: 266264. Strain: ch34 / atcc 43123 / dsm 2839. Gene: nbac, rmet_5193. Expressed in: escherichia coli. Expression_system_taxid: 562
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Resolution:
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1.84Å
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R-factor:
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0.207
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R-free:
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0.258
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Authors:
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F.Liu,A.Liu
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Key ref:
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F.Liu
and
a.liu
Probing extradiol dioxygenase mechanism in NAD+ biosy by viewing reaction cycle intermediates.
To be published,
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Date:
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19-Nov-14
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Release date:
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10-Feb-16
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PROCHECK
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Headers
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References
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Q1LCS4
(3HAO_CUPMC) -
3-hydroxyanthranilate 3,4-dioxygenase from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)
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Seq:
Struc:
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174 a.a.
174 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class:
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E.C.1.13.11.6
- 3-hydroxyanthranilate 3,4-dioxygenase.
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Pathway:
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(later stages)
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Reaction:
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3-hydroxyanthranilate + O2 = (2Z,4Z)-2-amino-3-carboxymuconate 6-semialdehyde
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3-hydroxyanthranilate
Bound ligand (Het Group name = )
matches with 84.62% similarity
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+
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O2
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=
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(2Z,4Z)-2-amino-3-carboxymuconate 6-semialdehyde
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Cofactor:
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Fe cation
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
