PDBsum entry 4v3c

Transferase

PDB id: 4v3c

Contents

Protein chain

388 a.a.

Ligands

__C

Waters ×297

PDB id:

4v3c

Name:

Transferase

Title:

The structure of alpha2,3-sialyltransferase variant 2 from pasteurella dagmatis in complex with the donor product cmp

Structure:

Sialyltransferase. Chain: a. Engineered: yes. Mutation: yes

Source:

Pasteurella dagmatis. Organism_taxid: 754. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.84Å R-factor: 0.175 R-free: 0.218

Authors:

T.Pavkov-Keller,K.Schmoelzer,T.Czabany,C.Luley-Goedl,D.Ribitsch, H.Schwab,B.Nidetzky,K.Gruber

Key ref:

K.Schmölzer et al. (2015). Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign. Chem Commun (camb), 51, 3083-3086. PubMed id: 25619424 DOI: 10.1039/c4cc09772f

Date:

17-Oct-14 Release date: 08-Apr-15

Protein chain

K9UUI6  (K9UUI6_9PAST) -  Sialyltransferase from Pasteurella dagmatis

Seq: 385 a.a.

Struc: 389 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DOI no: 10.1039/c4cc09772f

Chem Commun (camb) 51:3083-3086 (2015)

PubMed id: 25619424

Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign.

K.Schmölzer, T.Czabany, C.Luley-Goedl, T.Pavkov-Keller, D.Ribitsch, H.Schwab, K.Gruber, H.Weber, B.Nidetzky.

ABSTRACT

Structure-guided active-site redesign of a family GT-80 β-D-galactoside sialyltransferase (from Pasteurella dagmatis) to change enzyme regioselectivity from α-2,3 in the wild type to α-2,6 in a P7H-M117A double mutant is reported. Biochemical data for sialylation of lactose together with protein crystal structures demonstrate highly precise enzyme engineering.