PDBsum entry 4uqc

Hydrolase

PDB id: 4uqc

Contents

Protein chain

390 a.a.

Ligands

TAR

TLA

GLC

Waters ×582

PDB id:

4uqc

Name:

Hydrolase

Title:

X-ray structure of glucuronoxylan-xylanohydrolase (xyn30a) from clostridium thermocellum at 1.30 a resolution

Structure:

Carbohydrate binding family 6. Chain: a. Fragment: n-terminal catalytic module, residues 34-419. Synonym: xyn30a. Engineered: yes. Mutation: yes

Source:

Ruminiclostridium thermocellum. Organism_taxid: 1515. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

1.30Å R-factor: 0.113 R-free: 0.141

Authors:

F.Freire,A.K.Verma,A.Goyal,C.M.G.A.Fontes,S.Najmudin

Key ref:

F.Freire et al. (2016). Conservation in the mechanism of glucuronoxylan hydrolysis revealed by the structure of glucuronoxylan xylanohydrolase (CtXyn30A) from Clostridium thermocellum. Acta Crystallogr D Struct Biol, 72, 1162-1173. PubMed id: 27841749 DOI: 10.1107/S2059798316014376

Date:

22-Jun-14 Release date: 24-Jun-15

Protein chain

A3DJS9  (A3DJS9_CLOTH) -  Carbohydrate binding family 6 from Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372)

Seq: 630 a.a.

Struc: 390 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.8 - endo-1,4-beta-xylanase.
• Reaction: Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.

DOI no: 10.1107/S2059798316014376

Acta Crystallogr D Struct Biol 72:1162-1173 (2016)

PubMed id: 27841749

Conservation in the mechanism of glucuronoxylan hydrolysis revealed by the structure of glucuronoxylan xylanohydrolase (CtXyn30A) from Clostridium thermocellum.

F.Freire, A.Verma, P.Bule, V.D.Alves, C.M.Fontes, A.Goyal, S.Najmudin.

ABSTRACT

No abstract given.