PDBsum entry 4ufh

Hydrolase

PDB id: 4ufh

Contents

Protein chain

641 a.a.

Ligands

NAG-NAG ×3

GIF

PG4

NAG

Metals

_NI ×2

_CA

Waters ×214

PDB id:

4ufh

Name:

Hydrolase

Title:

Mouse galactocerebrosidase complexed with iso-galacto-fagomine igf

Structure:

Galactocerebrosidase. Chain: a. Synonym: galcerase, galactocerebroside beta-galactosidase, galactosylceramidase, galactosylceramide beta-galactosidase. Engineered: yes. Other_details: complex with igf

Source:

Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293t

Resolution:

2.16Å R-factor: 0.192 R-free: 0.216

Authors:

C.H.Hill,A.H.Viuff,S.J.Spratley,S.Salamone,S.H.Christensen,R.J.Read, N.W.Moriarty,H.H.Jensen,J.E.Deane

Key ref:

C.H.Hill et al. (2015). Azasugar inhibitors as pharmacological chaperones for Krabbe disease. Chem Sci, 6, 3075-3086. PubMed id: 26029356 DOI: 10.1039/c5sc00754b

Date:

17-Mar-15 Release date: 25-Mar-15

Protein chain

P54818  (GALC_MOUSE) -  Galactocerebrosidase from Mus musculus

Seq: 684 a.a.

Struc: 641 a.a.

Enzyme reactions

• Enzyme class: E.C.3.2.1.46 - galactosylceramidase.
• Reaction: a beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-galactose

beta-D-galactosyl-(1<->1')-N-acylsphing-4-enine + H2O = N-acylsphing-4-enine + D-galactose (Bound ligand (Het Group name = NAG) matches with 62.50% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1039/c5sc00754b

Chem Sci 6:3075-3086 (2015)

PubMed id: 26029356

Azasugar inhibitors as pharmacological chaperones for Krabbe disease.

C.H.Hill, A.H.Viuff, S.J.Spratley, S.Salamone, S.H.Christensen, R.J.Read, N.W.Moriarty, H.H.Jensen, J.E.Deane.

ABSTRACT

No abstract given.