PDBsum entry 4ufc

Hydrolase

PDB id: 4ufc

Contents

Protein chains

787 a.a.

Ligands

GIV ×2

CAC ×2

Metals

_CA ×3

PDB id:

4ufc

Name:

Hydrolase

Title:

Crystal structure of the gh95 enzyme bacova_03438

Structure:

Gh95. Chain: a, b. Engineered: yes

Source:

Bacteroides ovatus. Organism_taxid: 28116. Atcc: 8483. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.81Å R-factor: 0.188 R-free: 0.228

Authors:

A.Rogowski,J.A.Briggs,J.C.Mortimer,T.Tryfona,N.Terrapon,E.C.Lowe, A.Basle,C.Morland,A.M.Day,H.Zheng,T.E.Rogers,P.Thompson,A.R.Hawkins, M.P.Yadav,B.Henrissat,E.C.Martens,P.Dupree,H.J.Gilbert,D.N.Bolam

Key ref:

A.Rogowski et al. (2015). Glycan complexity dictates microbial resource allocation in the large intestine. Nat Commun, 6, 7481. PubMed id: 26112186 DOI: 10.1038/ncomms8481

Date:

16-Mar-15 Release date: 08-Jul-15

Protein chains

A7M011  (A7M011_BACO1) -  Glycosyl hydrolase family 95 N-terminal domain-containing protein from Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153)

Seq: 811 a.a.

Struc: 787 a.a.

DOI no: 10.1038/ncomms8481

Nat Commun 6:7481 (2015)

PubMed id: 26112186

Glycan complexity dictates microbial resource allocation in the large intestine.

A.Rogowski, J.A.Briggs, J.C.Mortimer, T.Tryfona, N.Terrapon, E.C.Lowe, A.Baslé, C.Morland, A.M.Day, H.Zheng, T.E.Rogers, P.Thompson, A.R.Hawkins, M.P.Yadav, B.Henrissat, E.C.Martens, P.Dupree, H.J.Gilbert, D.N.Bolam.

ABSTRACT

The structure of the human gut microbiota is controlled primarily through the degradation of complex dietary carbohydrates, but the extent to which carbohydrate breakdown products are shared between members of the microbiota is unclear. We show here, using xylan as a model, that sharing the breakdown products of complex carbohydrates by key members of the microbiota, such as Bacteroides ovatus, is dependent on the complexity of the target glycan. Characterization of the extensive xylan degrading apparatus expressed by B. ovatus reveals that the breakdown of the polysaccharide by the human gut microbiota is significantly more complex than previous models suggested, which were based on the deconstruction of xylans containing limited monosaccharide side chains. Our report presents a highly complex and dynamic xylan degrading apparatus that is fine-tuned to recognize the different forms of the polysaccharide presented to the human gut microbiota.