 |
PDBsum entry 4u3a
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Hydrolase
|
|
|
Title:
|
|
Crystal structure of ctcel5e
|
|
Structure:
|
|
Endoglucanase h. Chain: a, b. Fragment: unp residues 290-654. Synonym: cellulase h,endo-1,4-beta-glucanase h,egh, hcel5e. Engineered: yes
|
|
Source:
|
|
Clostridium thermocellum atcc 27405. Organism_taxid: 203119. Gene: celh, cthe_1472. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
|
|
Resolution:
|
|
|
2.42Å
|
R-factor:
|
0.204
|
R-free:
|
0.248
|
|
|
Authors:
|
|
S.F.Yuan,P.H.Liang,M.C.Ho
|
|
Key ref:
|
|
S.F.Yuan
et al.
(2015).
Biochemical characterization and structural analysis of a bifunctional cellulase/xylanase from Clostridium thermocellum.
J Biol Chem,
290,
5739-5748.
PubMed id:
DOI:
|
|
|
Date:
|
|
|
19-Jul-14
|
Release date:
|
14-Jan-15
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
P16218
(GUNH_CLOTH) -
Endoglucanase H from Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372)
|
|
|
|
Seq:
Struc:
|
|
|
|
900 a.a.
292 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
 |
PfamA domain |
|
|
 |
Secondary structure |
|
 |
CATH domain |
|
|
|
|
|
|
|
|
|
|
|
|
|
Enzyme class:
|
|
E.C.3.2.1.4
- cellulase.
|
|
|
|
|
|
|
Reaction:
|
|
Endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
DOI no:
|
J Biol Chem
290:5739-5748
(2015)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Biochemical characterization and structural analysis of a bifunctional cellulase/xylanase from Clostridium thermocellum.
|
|
S.F.Yuan,
T.H.Wu,
H.L.Lee,
H.Y.Hsieh,
W.L.Lin,
B.Yang,
C.K.Chang,
Q.Li,
J.Gao,
C.H.Huang,
M.C.Ho,
R.T.Guo,
P.H.Liang.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Links
