PDBsum entry 4ttt

Oxidoreductase

PDB id: 4ttt

Contents

Protein chains

598 a.a.

269 a.a.

Ligands

NFV

PO4

SF4

F3S

F4S

Metals

_NA

_CL ×3

_MG

Waters ×614

PDB id:

4ttt

Name:

Oxidoreductase

Title:

Crystal structure of an o2-tolerant [nife]-hydrogenase from ralstonia eutropha in its as-isolated form - oxidized state 3

Structure:

Uptake hydrogenase large subunit hoxg. Chain: l. Fragment: unp residues 1-603. Synonym: hydrogenlyase,membrane-bound hydrogenase large subunit. Engineered: yes. Uptake hydrogenase small subunit hoxk. Chain: s. Fragment: residues 44-360. Synonym: hydrogenlyase,membrane-bound hydrogenase small subunit.

Source:

Ralstonia eutropha. Organism_taxid: 381666. Strain: atcc 17699 / h16 / dsm 428 / stanier 337. Atcc: 17699. Gene: hoxg, phg002. Expressed in: ralstonia eutropha. Expression_system_taxid: 381666. Expression_system_atcc_number: 17699. Gene: hoxk, phg001.

Resolution:

1.72Å R-factor: 0.136 R-free: 0.165

Authors:

A.Schmidt,J.Kalms,P.Scheerer

Key ref:

A.Schmidt et al. Crystal structure of an o2-Tolerant [nife]-Hydrogenas ralstonia eutropha in its as-Isolated form (oxidized - 3. To be published, .

Date:

23-Jun-14 Release date: 28-Jan-15

Protein chain

P31891  (MBHL_CUPNH) -  Uptake hydrogenase large subunit from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337)

Seq: 618 a.a.

Struc: 598 a.a.

Protein chain

P31892  (MBHS_CUPNH) -  Uptake hydrogenase small subunit from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337)

Seq: 360 a.a.

Struc: 269 a.a.

Enzyme reactions

• Enzyme class: Chains L, S: E.C.1.12.99.6 - hydrogenase (acceptor).
• Reaction: H2 + A = AH2
• Cofactor: Iron-sulfur; Ni(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site