PDBsum entry 4tq7

Structural protein

PDB id: 4tq7

Contents

Protein chains

140 a.a.

Waters ×84

PDB id:

4tq7

Name:

Structural protein

Title:

N-terminal domain of c. Reinhardtii sas-6 homolog bld12p q93e (nn10)

Structure:

Centriole protein. Chain: a, b. Synonym: n-terminal domain of c. Reinhardtii sas-6 homolog bld12p q93e (nn10). Engineered: yes. Mutation: yes

Source:

Chlamydomonas reinhardtii. Organism_taxid: 3055. Gene: crsas-6. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

2.64Å R-factor: 0.210 R-free: 0.258

Authors:

M.Hilbert,S.H.W.Kraatz

Key ref:

M.Hilbert et al. (2016). SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture. Nat Cell Biol, 18, 393-403. PubMed id: 26999736 DOI: 10.1038/ncb3329

Date:

10-Jun-14 Release date: 24-Jun-15

Protein chains

A9CQL4  (A9CQL4_CHLRE) -  Centriole protein from Chlamydomonas reinhardtii

Seq: 728 a.a.

Struc: 140 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1038/ncb3329

Nat Cell Biol 18:393-403 (2016)

PubMed id: 26999736

SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture.

M.Hilbert, A.Noga, D.Frey, V.Hamel, P.Guichard, S.H.Kraatz, M.Pfreundschuh, S.Hosner, I.Flückiger, R.Jaussi, M.M.Wieser, K.M.Thieltges, X.Deupi, D.J.Müller, R.A.Kammerer, P.Gönczy, M.Hirono, M.O.Steinmetz.

ABSTRACT

Centrioles are critical for the formation of centrosomes, cilia and flagella in eukaryotes. They are thought to assemble around a nine-fold symmetric cartwheel structure established by SAS-6 proteins. Here, we have engineered Chlamydomonas reinhardtii SAS-6-based oligomers with symmetries ranging from five- to ten-fold. Expression of a SAS-6 mutant that forms six-fold symmetric cartwheel structures in vitro resulted in cartwheels and centrioles with eight- or nine-fold symmetries in vivo. In combination with Bld10 mutants that weaken cartwheel-microtubule interactions, this SAS-6 mutant produced six- to eight-fold symmetric cartwheels. Concurrently, the microtubule wall maintained eight- and nine-fold symmetries. Expressing SAS-6 with analogous mutations in human cells resulted in nine-fold symmetric centrioles that exhibited impaired length and organization. Together, our data suggest that the self-assembly properties of SAS-6 instruct cartwheel symmetry, and lead us to propose a model in which the cartwheel and the microtubule wall assemble in an interdependent manner to establish the native architecture of centrioles.