PDBsum entry 4rv3

Lyase

PDB id: 4rv3

Contents

Protein chain

302 a.a.

Ligands

ACT

INS

Waters ×161

PDB id:

4rv3

Name:

Lyase

Title:

Crystal structure of a pentafluoro-phe incorporated phosphatidylinositol-specific phospholipasE C (h258x)from staphylococcus aureus

Structure:

1-phosphatidylinositol phosphodiesterase. Chain: a. Engineered: yes. Mutation: yes

Source:

Staphylococcus aureus. Organism_taxid: 426430. Strain: newman. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.00Å R-factor: 0.183 R-free: 0.238

Authors:

T.He,A.Gershenson,S.J.Eyles,J.Gao,M.F.Roberts

Key ref:

T.He et al. (2015). Fluorinated Aromatic Amino Acids Distinguish Cation-π Interactions from Membrane Insertion. J Biol Chem, 290, 19334-19342. PubMed id: 26092728 DOI: 10.1074/jbc.M115.668343

Date:

24-Nov-14 Release date: 01-Jul-15

Protein chain

P45723  (PLC_STAAE) -  1-phosphatidylinositol phosphodiesterase from Staphylococcus aureus (strain Newman)

Seq: 312 a.a.

Struc: 302 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.6.1.13 - phosphatidylinositol diacylglycerol-lyase.
• Pathway: 1-Phosphatidyl-myo-inositol Metabolism
• Reaction: a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) = 1D-myo-inositol 1,2-cyclic phosphate + a 1,2-diacyl-sn-glycerol

1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) = 1D-myo-inositol 1,2-cyclic phosphate (Bound ligand (Het Group name = INS) matches with 80.00% similarity) + 1,2-diacyl-sn-glycerol

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1074/jbc.M115.668343

J Biol Chem 290:19334-19342 (2015)

PubMed id: 26092728

Fluorinated Aromatic Amino Acids Distinguish Cation-π Interactions from Membrane Insertion.

T.He, A.Gershenson, S.J.Eyles, Y.J.Lee, W.R.Liu, J.Wang, J.Gao, M.F.Roberts.

ABSTRACT

No abstract given.