PDBsum entry 4pwc

Oxidoreductase

PDB id: 4pwc

Contents

Protein chain

490 a.a.

Ligands

FAD

Metals

_BR ×68

_NA ×2

Waters ×150

PDB id:

4pwc

Name:

Oxidoreductase

Title:

Phl p 4 i153v n158h variant, a glucose oxidase, 3.5 m nabr soak

Structure:

Pollen allergen phl p 4.0202. Chain: a. Fragment: phl p 4.0202. Engineered: yes. Mutation: yes

Source:

Phleum pratense. Timothy. Organism_taxid: 15957. Gene: phlp4. Expressed in: komagataella pastoris. Expression_system_taxid: 4922

Resolution:

2.30Å R-factor: 0.188 R-free: 0.225

Authors:

D.Zafred,W.Keller,P.Macheroux

Key ref:

D.Zafred et al. (2015). Rationally engineered flavin-dependent oxidase reveals steric control of dioxygen reduction. Febs J, 282, 3060-3074. PubMed id: 25619330 DOI: 10.1111/febs.13212

Date:

19-Mar-14 Release date: 02-Apr-14

Protein chain

B2ZWE9  (B2ZWE9_PHLPR) -  Pollen allergen Phl p 4.0202 (Fragment) from Phleum pratense

Seq: 500 a.a.

Struc: 490 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

DOI no: 10.1111/febs.13212

Febs J 282:3060-3074 (2015)

PubMed id: 25619330

Rationally engineered flavin-dependent oxidase reveals steric control of dioxygen reduction.

D.Zafred, B.Steiner, A.R.Teufelberger, A.Hromic, P.A.Karplus, C.J.Schofield, S.Wallner, P.Macheroux.

ABSTRACT

No abstract given.