PDBsum entry 4owc

Hydrolase

PDB id: 4owc

Contents

Protein chain

129 a.a.

Metals

_NA

IOD ×8

_PT ×2

_CL ×4

Waters ×105

PDB id:

4owc

Name:

Hydrolase

Title:

Pti6 binding to hewl

Structure:

LysozymE C. Chain: a. Synonym: 1,4-beta-n-acetylmuramidasE C, allergen gal d iv. Ec: 3.2.1.17

Source:

Gallus gallus. Chicken. Organism_taxid: 9031

Resolution:

1.62Å R-factor: 0.175 R-free: 0.193

Authors:

S.W.M.Tanley,V.L.Starkey,L.Lamplough,S.Kaenket,J.R.Helliwell

Key ref:

S.W.Tanley et al. (2014). The binding of platinum hexahalides (Cl, Br and I) to hen egg-white lysozyme and the chemical transformation of the PtI6 octahedral complex to a PtI3 moiety bound to His15. Acta Crystallogr F Struct Biol Commun, 70, 1132-1134. PubMed id: 25195880 DOI: 10.1107/S2053230X14014009

Date:

31-Jan-14 Release date: 24-Sep-14

Protein chain

P00698  (LYSC_CHICK) -  Lysozyme C from Gallus gallus

Seq: 147 a.a.

Struc: 129 a.a.

Enzyme reactions

• Enzyme class: E.C.3.2.1.17 - lysozyme.
• Reaction: Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.

DOI no: 10.1107/S2053230X14014009

Acta Crystallogr F Struct Biol Commun 70:1132-1134 (2014)

PubMed id: 25195880

The binding of platinum hexahalides (Cl, Br and I) to hen egg-white lysozyme and the chemical transformation of the PtI6 octahedral complex to a PtI3 moiety bound to His15.

S.W.Tanley, L.V.Starkey, L.Lamplough, S.Kaenket, J.R.Helliwell.

ABSTRACT

This study examines the binding and chemical stability of the platinum hexahalides K2PtCl6, K2PtBr6 and K2PtI6 when soaked into pre-grown hen egg-white lysozyme (HEWL) crystals as the protein host. Direct comparison of the iodo complex with the chloro and bromo complexes shows that the iodo complex is partly chemically transformed to a square-planar PtI3 complex bound to the N(δ) atom of His15, a chemical behaviour that is not exhibited by the chloro or bromo complexes. Each complex does, however, bind to HEWL in its octahedral form either at one site (PtI6) or at two sites (PtBr6 and PtCl6). As heavy-atom derivatives of a protein, the octahedral shape of the hexahalides could be helpful in cases of difficult-to-interpret electron-density maps as they would be recognisable `objects'.