PDBsum entry 4ofh

Hydrolase/DNA

PDB id: 4ofh

Contents

Protein chain

141 a.a.

DNA/RNA

Metals

_MG

Waters ×91

PDB id:

4ofh

Name:

Hydrolase/DNA

Title:

Structural basis for thymine glycosylase activity on t:o6-methylg mismatch by methyl-cpg binding domain protein 4: implications for roles of arg468 in mismatch recognition and catalysis

Structure:

Methyl-cpg-binding domain protein 4. Chain: a. Fragment: catalytic domain of mbd4. Synonym: methyl-cpg-binding endonuclease 1, methyl-cpg-binding protein mbd4, mismatch-specific DNA n-glycosylase. Engineered: yes. Mutation: yes. 12-mer DNA(t). Chain: c.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mbd4, med1. Expressed in: escherichia coli. Expression_system_taxid: 511693. Synthetic: yes. Other_details: nature substrate. Other_details: nature substrate

Resolution:

2.22Å R-factor: 0.188 R-free: 0.230

Authors:

H.Ouzon-Shubeita,Y.-L.Lin,S.Lee

Key ref:

H.Ouzon-Shubeita et al. Structure of mbd4 bound to o6meg:t mispair DNA. To be published, .

Date:

14-Jan-14 Release date: 22-Apr-15

Protein chain

O95243  (MBD4_HUMAN) -  Methyl-CpG-binding domain protein 4 from Homo sapiens

Seq: 580 a.a.

Struc: 141 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

C-C-A-G-C-G-T-G-C-A-G-C 12 bases

G-C-T-G-C-6OG-C-G-C-T-G-G 12 bases

Enzyme reactions

• Enzyme class: E.C.3.2.2.- - ?????