PDBsum entry 4n8s

Hydrolase

PDB id: 4n8s

Contents

Protein chains

269 a.a.

Ligands

NAG ×2

XXH ×2

EAC ×2

GOL

Waters ×163

PDB id:

4n8s

Name:

Hydrolase

Title:

Crystal structure of the ternary complex of lipase from thermomyces lanuginosa with ethylacetoacetate and p-nitrobenzaldehyde at 2.3 a resolution

Structure:

Lipase. Chain: a, b. Synonym: triacylglycerol lipase. Ec: 3.1.1.3

Source:

Thermomyces lanuginosus. Organism_taxid: 5541

Resolution:

2.30Å R-factor: 0.223 R-free: 0.266

Authors:

M.Kumar,J.Mukherjee,M.N.Gupta,M.Sinha,P.Kaur,S.Sharma,T.P.Singh

Key ref:

M.Kumar et al. Crystal structure of the ternary complex of lipase fr thermomyces lanuginosa with ethylacetoacetate and p-Nitrobenzaldehyde at 2.3 a resolution. To be published, .

Date:

18-Oct-13 Release date: 06-Nov-13

Protein chains

O59952  (LIP_THELA) -  Lipase from Thermomyces lanuginosus

Seq: 291 a.a.

Struc: 269 a.a.

Enzyme reactions

• Enzyme class: E.C.3.1.1.3 - triacylglycerol lipase.
• Reaction: a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H⁺

triacylglycerol (Bound ligand (Het Group name = GOL) matches with 40.00% similarity) + H2O = diacylglycerol (Bound ligand (Het Group name = EAC) matches with 40.00% similarity) + fatty acid + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site