PDBsum entry 4mpp

Lyase

PDB id: 4mpp

Contents

Protein chain

525 a.a.

Ligands

TZD

GOL ×3

Metals

_CL

_CA ×2

_NA ×2

Waters ×572

PDB id:

4mpp

Name:

Lyase

Title:

Crystal structure of benzoylformate decarboxylase mutant h281y/t377p/f397t/a460i

Structure:

Benzoylformate decarboxylase. Chain: a. Synonym: bfd, bfdc. Engineered: yes. Mutation: yes

Source:

Pseudomonas putida. Organism_taxid: 303. Gene: mdlc. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.50Å R-factor: 0.134 R-free: 0.159

Authors:

F.H.Andrews,M.J.Mcleish

Key ref:

F.H.Andrews et al. A pseudo study of thiamin diphosphate-Dependent decarboxylases. To be published, .

Date:

13-Sep-13 Release date: 18-Mar-15

Protein chain

P20906  (MDLC_PSEPU) -  Benzoylformate decarboxylase from Pseudomonas putida

Seq: 528 a.a.

Struc: 525 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.4.1.1.7 - benzoylformate decarboxylase.
• Reaction: phenylglyoxylate + H⁺ = benzaldehyde + CO2

phenylglyoxylate + H(+) (Bound ligand (Het Group name = GOL) matches with 41.67% similarity) = benzaldehyde + CO2

• Cofactor: Thiamine diphosphate

Thiamine diphosphate (Bound ligand (Het Group name = TZD) matches with 96.30% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site