PDBsum entry 4moo

Oxidoreductase

PDB id: 4moo

Contents

Protein chain

576 a.a.

Ligands

FDA

GAF

Waters ×514

PDB id:

4moo

Name:

Oxidoreductase

Title:

Pyranose 2-oxidase h450g mutant with 2-fluorinated galactose

Structure:

Pyranose 2-oxidase. Chain: a. Synonym: pyranose oxidase. Engineered: yes. Mutation: yes

Source:

Trametes ochracea. White-rot fungus. Organism_taxid: 230624. Gene: p2o. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.65Å R-factor: 0.192 R-free: 0.224

Authors:

T.C.Tan,O.Spadiut,R.Gandini,D.Haltrich,C.Divne

Date:

12-Sep-13 Release date: 05-Feb-14

Protein chain

Q7ZA32  (Q7ZA32_TRAOC) -  Pyranose 2-oxidase from Trametes ochracea

Seq: 623 a.a.

Struc: 576 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.1.3.10 - pyranose oxidase.
• Reaction: D-glucose + O2 = 2-dehydro-D-glucose + H2O2

D-glucose (Bound ligand (Het Group name = GAF) matches with 84.62% similarity) + O2 = 2-dehydro-D-glucose + H2O2

• Cofactor: FAD

FAD (Bound ligand (Het Group name = FDA) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site