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PDBsum entry 4mc2
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Hydrolase/hydrolase inhibitor
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PDB id
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4mc2
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PDB id:
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| Name: |
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Hydrolase/hydrolase inhibitor
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Title:
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HIV protease in complex with sa525p
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Structure:
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Protease. Chain: a, b. Fragment: unp residues 489-587. Synonym: pr, retropepsin. Engineered: yes. Mutation: yes
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Source:
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Human immunodeficiency virus 1. HIV-1. Organism_taxid: 11676. Gene: gag-pol. Expressed in: escherichia coli. Expression_system_taxid: 562
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Resolution:
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1.56Å
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R-factor:
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0.200
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R-free:
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0.207
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Authors:
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A.K.Ganguly,S.S.Alluri,C.Wang,D.Caroccia,D.Biswas,E.Kang,L.Zhang, S.S.Carroll,C.Burlein,V.Munshi,P.Orth,C.Strickland
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Key ref:
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A.K.Ganguly
et al.
Structural optimization of cyclic sulfonamide based n HIV-1 protease inhibitors to pico molar affinities gu X-Ray crystallographic analysis.
Tetrahedron,
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Date:
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21-Aug-13
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Release date:
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02-Apr-14
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PROCHECK
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Headers
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References
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P0C6F2
(POL_HV1LW) -
Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate LW123)
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Seq:
Struc:
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1435 a.a.
99 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 3 residue positions (black
crosses)
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Enzyme class 1:
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E.C.2.7.7.-
- ?????
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Enzyme class 2:
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E.C.2.7.7.49
- RNA-directed Dna polymerase.
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Reaction:
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DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
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DNA(n)
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+
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2'-deoxyribonucleoside 5'-triphosphate
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=
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DNA(n+1)
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+
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diphosphate
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Enzyme class 3:
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E.C.2.7.7.7
- DNA-directed Dna polymerase.
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Reaction:
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DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
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DNA(n)
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+
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2'-deoxyribonucleoside 5'-triphosphate
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=
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DNA(n+1)
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+
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diphosphate
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Enzyme class 4:
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E.C.3.1.-.-
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Enzyme class 5:
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E.C.3.1.13.2
- exoribonuclease H.
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Reaction:
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Exonucleolytic cleavage to 5'-phosphomonoester oligonucleotides in both 5'- to 3'- and 3'- to 5'-directions.
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Enzyme class 6:
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E.C.3.1.26.13
- retroviral ribonuclease H.
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Enzyme class 7:
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E.C.3.4.23.16
- HIV-1 retropepsin.
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Reaction:
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Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
