PDBsum entry 4m1e

Transferase

PDB id: 4m1e

Contents

Protein chains

(+ 0 more) 272 a.a.

Ligands

SO4 ×6

ADE ×6

6PC ×6

Waters ×662

PDB id:

4m1e

Name:

Transferase

Title:

Crystal structure of purine nucleoside phosphorylase i from planctomyces limnophilus dsm 3776, nysgrc target 029364.

Structure:

Purine nucleoside phosphorylase. Chain: a, b, c, e, d, f. Synonym: inosine-guanosine phosphorylase. Engineered: yes

Source:

Planctomyces limnophilus. Organism_taxid: 521674. Strain: dsm 3776. Gene: plim_2216. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.90Å R-factor: 0.215 R-free: 0.258

Authors:

V.N.Malashkevich,J.B.Bonanno,R.Bhosle,R.Toro,B.Hillerich,A.Gizzi, S.Garforth,A.Kar,M.K.Chan,J.Lafluer,H.Patel,B.Matikainen,S.Chamala, S.Lim,A.Celikgil,G.Villegas,B.Evans,J.Love,A.Fiser,K.Khafizov, R.Seidel,S.C.Almo,New York Structural Genomics Research Consortium (Nysgrc)

Key ref:

V.N.Malashkevich et al. Crystal structure of purine nucleoside phosphorylase planctomyces limnophilus dsm 3776, Nysgrc target 0293. To be published, .

Date:

02-Aug-13 Release date: 21-Aug-13

Protein chains

D5SMY7  (D5SMY7_PLAL2) -  Purine nucleoside phosphorylase from Planctopirus limnophila (strain ATCC 43296 / DSM 3776 / IFAM 1008 / Mu 290)

Seq: 275 a.a.

Struc: 272 a.a.

Enzyme reactions

• Enzyme class: E.C.2.4.2.1 - purine-nucleoside phosphorylase.
• Reaction:
  1. a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha- D-ribose 1-phosphate
  2. a purine 2'-deoxy-D-ribonucleoside + phosphate = a purine nucleobase + 2-deoxy-alpha-D-ribose 1-phosphate

purine D-ribonucleoside + phosphate = purine nucleobase + alpha- D-ribose 1-phosphate (Bound ligand (Het Group name = 6PC) matches with 43.75% similarity)

purine 2'-deoxy-D-ribonucleoside + phosphate = purine nucleobase (Bound ligand (Het Group name = 6PC) matches with 46.67% similarity) + 2-deoxy-alpha-D-ribose 1-phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site