PDBsum entry 4lop

Transferase

PDB id: 4lop

Contents

Protein chains

351 a.a.

21 a.a.

18 a.a.

19 a.a.

Ligands

SB4 ×4

TLA ×2

EDO ×15

Waters ×865

PDB id:

4lop

Name:

Transferase

Title:

Structural basis of autoactivation of p38 alpha induced by tab1 (tetragonal crystal form)

Structure:

Mitogen-activated protein kinase 14. Chain: a, b, c, d. Fragment: kinase domain (1-360). Synonym: map kinase 14, mapk 14, crk1, mitogen-activated protein kinase p38 alpha, map kinase p38 alpha. Engineered: yes. Tgf-beta-activated kinase 1 and map3k7-binding protein 1. Chain: k, l, m, n. Fragment: residues 384-412.

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: mapk14, crk1, csbp1, csbp2. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: synthetic peptide

Resolution:

2.05Å R-factor: 0.150 R-free: 0.188

Authors:

A.Chaikuad,G.F.Denicola,T.Krojer,C.K.Allerston,F.Von Delft, C.H.Arrowsmith,A.M.Edwards,C.Bountra,M.S.Marber,S.Knapp,Structural Genomics Consortium (Sgc)

Key ref:

G.F.De Nicola et al. (2013). Mechanism and consequence of the autoactivation of p38α mitogen-activated protein kinase promoted by TAB1. Nat Struct Biol, 20, 1182-1190. PubMed id: 24037507 DOI: 10.1038/nsmb.2668

Date:

13-Jul-13 Release date: 21-Aug-13

Protein chains

P47811  (MK14_MOUSE) -  Mitogen-activated protein kinase 14 from Mus musculus

Seq: 360 a.a.

Struc: 351 a.a.

Protein chains

Q8CF89  (TAB1_MOUSE) -  TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 from Mus musculus

Seq: 502 a.a.

Struc: 21 a.a.

Protein chain

Q8CF89  (TAB1_MOUSE) -  TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 from Mus musculus

Seq: 502 a.a.

Struc: 18 a.a.

Protein chain

Q8CF89  (TAB1_MOUSE) -  TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 from Mus musculus

Seq: 502 a.a.

Struc: 19 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.2.7.11.24 - mitogen-activated protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1038/nsmb.2668

Nat Struct Biol 20:1182-1190 (2013)

PubMed id: 24037507

Mechanism and consequence of the autoactivation of p38α mitogen-activated protein kinase promoted by TAB1.

G.F.De Nicola, E.D.Martin, A.Chaikuad, R.Bassi, J.Clark, L.Martino, S.Verma, P.Sicard, R.Tata, R.A.Atkinson, S.Knapp, M.R.Conte, M.S.Marber.

ABSTRACT

No abstract given.