PDBsum entry 4lga

Hydrolase/receptor

PDB id: 4lga

Contents

Protein chains

176 a.a.

303 a.a.

Ligands

A2O

SO4 ×2

Metals

_MG ×2

Waters ×73

PDB id:

4lga

Name:

Hydrolase/receptor

Title:

Aba-mimicking ligand n-(2-oxo-1-propyl-1,2,3,4-tetrahydroquinolin-6- yl)-1-phenylmethanesulfonamide in complex with aba receptor pyl2 and pp2c hab1

Structure:

Abscisic acid receptor pyl2. Chain: a. Fragment: unp residues 14-188. Synonym: pyr1-like protein 2, regulatory components of aba receptor 14. Engineered: yes. Protein phosphatase 2c 16. Chain: b. Fragment: unp residues 172-511.

Source:

Arabidopsis thaliana. Mouse-ear cress,thale-cress. Organism_taxid: 3702. Gene: pyl2, rcar14, at2g26040, t19l18.15. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: hab1, p2c-ha, at1g72770, f28p22.4.

Resolution:

2.70Å R-factor: 0.250 R-free: 0.280

Authors:

X.E.Zhou,M.Gao,X.Liu,Y.Zhang,X.Xue,K.Melcher,P.Gao,F.Wang,L.Zeng, Y.Zhao,Y.Zhao,P.Deng,D.Zhong,J.-K.Zhu,Y.Xu,H.E.Xu

Key ref:

M.Cao et al. (2013). An ABA-mimicking ligand that reduces water loss and promotes drought resistance in plants. Cell Res, 23, 1043-1054. PubMed id: 23835477 DOI: 10.1038/cr.2013.95

Date:

27-Jun-13 Release date: 14-Aug-13

Protein chain

O80992  (PYL2_ARATH) -  Abscisic acid receptor PYL2 from Arabidopsis thaliana

Seq: 190 a.a.

Struc: 176 a.a.*

Protein chain

Q9CAJ0  (P2C16_ARATH) -  Protein phosphatase 2C 16 from Arabidopsis thaliana

Seq: 511 a.a.

Struc: 303 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain B: E.C.3.1.3.16 - protein-serine/threonine phosphatase.
• Reaction:
  1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
  2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1038/cr.2013.95

Cell Res 23:1043-1054 (2013)

PubMed id: 23835477

An ABA-mimicking ligand that reduces water loss and promotes drought resistance in plants.

M.Cao, X.Liu, Y.Zhang, X.Xue, X.E.Zhou, K.Melcher, P.Gao, F.Wang, L.Zeng, Y.Zhao, Y.Zhao, P.Deng, D.Zhong, J.K.Zhu, H.E.Xu, Y.Xu.

ABSTRACT

Abscisic acid (ABA) is the most important hormone for plants to resist drought and other abiotic stresses. ABA binds directly to the PYR/PYL family of ABA receptors, resulting in inhibition of type 2C phosphatases (PP2C) and activation of downstream ABA signaling. It is envisioned that intervention of ABA signaling by small molecules could help plants to overcome abiotic stresses such as drought, cold and soil salinity. However, chemical instability and rapid catabolism by plant enzymes limit the practical application of ABA itself. Here we report the identification of a small molecule ABA mimic (AM1) that acts as a potent activator of multiple members of the family of ABA receptors. In Arabidopsis, AM1 activates a gene network that is highly similar to that induced by ABA. Treatments with AM1 inhibit seed germination, prevent leaf water loss, and promote drought resistance. We solved the crystal structure of AM1 in complex with the PYL2 ABA receptor and the HAB1 PP2C, which revealed that AM1 mediates a gate-latch-lock interacting network, a structural feature that is conserved in the ABA-bound receptor/PP2C complex. Together, these results demonstrate that a single small molecule ABA mimic can activate multiple ABA receptors and protect plants from water loss and drought stress. Moreover, the AM1 complex crystal structure provides a structural basis for designing the next generation of ABA-mimicking small molecules.