PDBsum entry 4lg2

RNA binding protein/RNA

PDB id: 4lg2

Contents

Protein chains

122 a.a.

DNA/RNA

Waters ×8

PDB id:

4lg2

Name:

RNA binding protein/RNA

Title:

Crystal structure of reston ebola virus vp35 RNA binding domain bound to 12-bp dsrna

Structure:

Polymerase cofactor. Chain: a, b, c, d. Fragment: unp residues 205-329. Engineered: yes. Dsrna. Chain: e, f, j, i. Engineered: yes

Source:

Reston ebolavirus. Rebov. Organism_taxid: 386032. Strain: reston. Gene: vp35, rebovgp2. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes

Resolution:

2.70Å R-factor: 0.211 R-free: 0.262

Authors:

S.Bale,J.-P.Julien,Z.A.Bornholdt,A.S.Krois,I.A.Wilson,E.O.Saphire

Key ref:

S.Bale et al. (2013). Ebolavirus VP35 coats the backbone of double-stranded RNA for interferon antagonism. J Virol, 87, 10385-10388. PubMed id: 23824825 DOI: 10.1128/JVI.01452-13

Date:

27-Jun-13 Release date: 10-Jul-13

Protein chains

Q8JPY0  (VP35_EBORR) -  Polymerase cofactor VP35 from Reston ebolavirus (strain Reston-89)

Seq: 329 a.a.

Struc: 122 a.a.

DNA/RNA chains

C-U-A-G-A-C-G-U-C 9 bases

G-A-C-G-U-C-U-A-G 9 bases

C-U-A-G-A-C-G-U-C-U-A-G 12 bases

C-U-A-G-A-C-G-U-C-U-A-G 12 bases

DOI no: 10.1128/JVI.01452-13

J Virol 87:10385-10388 (2013)

PubMed id: 23824825

Ebolavirus VP35 coats the backbone of double-stranded RNA for interferon antagonism.

S.Bale, J.P.Julien, Z.A.Bornholdt, A.S.Krois, I.A.Wilson, E.O.Saphire.

ABSTRACT

Recognition of viral double-stranded RNA (dsRNA) activates interferon production and immune signaling in host cells. Crystal structures of ebolavirus VP35 show that it caps dsRNA ends to prevent sensing by pattern recognition receptors such as RIG-I. In contrast, structures of marburgvirus VP35 show that it primarily coats the dsRNA backbone. Here, we demonstrate that ebolavirus VP35 also coats the dsRNA backbone in solution, although binding to the dsRNA ends probably constitutes the initial binding event.