PDBsum entry 4lfh

Immune system

PDB id: 4lfh

Contents

Protein chains

202 a.a.

222 a.a.

Ligands

NAG-NAG

ACT

Metals

_NA ×2

Waters ×215

PDB id:

4lfh

Name:

Immune system

Title:

Crystal structure of 9c2 tcr

Structure:

9c2 tcr delta chain. Chain: d. Engineered: yes. 9c2 tcr gamma chain. Chain: g. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293.

Resolution:

2.30Å R-factor: 0.189 R-free: 0.238

Authors:

A.P.Uldrich,J.Le Nours,D.G.Pellicci,S.Gras,J.Rossjohn,D.I.Godfrey

Key ref:

A.P.Uldrich et al. (2013). CD1d-lipid antigen recognition by the γδ TCR. Nat Immunol, 14, 1137-1145. PubMed id: 24076636 DOI: 10.1038/ni.2713

Date:

26-Jun-13 Release date: 02-Oct-13

Protein chain

Q6PJ56  (Q6PJ56_HUMAN) -  TRA@ protein from Homo sapiens

Seq: 296 a.a.

Struc: 202 a.a.*

Protein chain

P0CF51  (TRGC1_HUMAN) -  T cell receptor gamma constant 1 from Homo sapiens

Seq: 173 a.a.

Struc: 222 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

DOI no: 10.1038/ni.2713

Nat Immunol 14:1137-1145 (2013)

PubMed id: 24076636

CD1d-lipid antigen recognition by the γδ TCR.

A.P.Uldrich, J.Le Nours, D.G.Pellicci, N.A.Gherardin, K.G.McPherson, R.T.Lim, O.Patel, T.Beddoe, S.Gras, J.Rossjohn, D.I.Godfrey.

ABSTRACT

The T cell repertoire comprises αβ and γδ T cell lineages. Although it is established how αβ T cell antigen receptors (TCRs) interact with antigen presented by antigen-presenting molecules, this is unknown for γδ TCRs. We describe a population of human Vδ1(+) γδ T cells that exhibit autoreactivity to CD1d and provide a molecular basis for how a γδ TCR binds CD1d-α-galactosylceramide (α-GalCer). The γδ TCR docked orthogonally, over the A' pocket of CD1d, in which the Vδ1-chain, and in particular the germ line-encoded CDR1δ loop, dominated interactions with CD1d. The TCR γ-chain sat peripherally to the interface, with the CDR3γ loop representing the principal determinant for α-GalCer specificity. Accordingly, we provide insight into how a γδ TCR binds specifically to a lipid-loaded antigen-presenting molecule.