PDBsum entry 4l6y

Structural protein

PDB id: 4l6y

Contents

Protein chain

445 a.a.

PDB id:

4l6y

Name:

Structural protein

Title:

Structure of the microtubule associated protein prc1 (protein regulator of cytokinesis 1)

Structure:

Protein regulator of cytokinesis 1. Chain: a, b. Fragment: unp residues 1-486. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: prc1. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

3.30Å R-factor: 0.276 R-free: 0.306

Authors:

R.Subramanian,S.Ti,L.Tan,S.A.Darst,T.M.Kapoor

Key ref:

R.Subramanian et al. (2013). Marking and measuring single microtubules by PRC1 and kinesin-4. Cell, 154, 377-390. PubMed id: 23870126 DOI: 10.1016/j.cell.2013.06.021

Date:

13-Jun-13 Release date: 17-Jul-13

Protein chains

O43663  (PRC1_HUMAN) -  Protein regulator of cytokinesis 1 from Homo sapiens

Seq: 620 a.a.

Struc: 445 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1016/j.cell.2013.06.021

Cell 154:377-390 (2013)

PubMed id: 23870126

Marking and measuring single microtubules by PRC1 and kinesin-4.

R.Subramanian, S.C.Ti, L.Tan, S.A.Darst, T.M.Kapoor.

ABSTRACT

Error-free cell division depends on the assembly of the spindle midzone, a specialized array of overlapping microtubules that emerges between segregating chromosomes during anaphase. The molecular mechanisms by which a subset of dynamic microtubules from the metaphase spindle are selected and organized into a stable midzone array are poorly understood. Here, we show using in vitro reconstitution assays that PRC1 and kinesin-4, two microtubule-associated proteins required for midzone assembly, can tag microtubule plus ends. Remarkably, the size of these tags is proportional to filament length. We determine the crystal structure of the PRC1 homodimer and map the protein-protein interactions needed for tagging microtubule ends. Importantly, length-dependent microtubule plus-end-tagging by PRC1 is also observed in dividing cells. Our findings suggest how biochemically similar microtubules can be differentially marked, based on length, for selective regulation during the formation of specialized arrays, such as those required for cytokinesis.