PDBsum entry 4jwt

Hydrolase

PDB id: 4jwt

Contents

Protein chain

245 a.a.

Ligands

EDO ×4

ADE

Waters ×141

PDB id:

4jwt

Name:

Hydrolase

Title:

Crystal structure of a putative 5'-methylthioadenosine/s- adenosylhomocysteine nucleosidase from sulfurimonas denitrificans dsm 1251 (target nysgrc-029304 )

Structure:

Methylthioadenosine nucleosidase. Chain: a. Engineered: yes

Source:

Sulfurimonas denitrificans. Organism_taxid: 326298. Strain: dsm 1251. Gene: suden_1392. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.05Å R-factor: 0.178 R-free: 0.193

Authors:

P.Sampathkumar,S.C.Almo,New York Structural Genomics Research Consortium (Nysgrc)

Key ref:

P.Sampathkumar et al. Crystal structure of a putative 5'-Methylthioadenosine/s-Adenosylhomocysteine nucleos from sulfurimonas denitrificans dsm 1251. To be published, .

Date:

27-Mar-13 Release date: 08-May-13

Protein chain

Q30QR2  (Q30QR2_SULDN) -  adenosylhomocysteine nucleosidase from Sulfurimonas denitrificans (strain ATCC 33889 / DSM 1251)

Seq: 237 a.a.

Struc: 245 a.a.

Enzyme reactions

• Enzyme class: E.C.3.2.2.9 - adenosylhomocysteine nucleosidase.
• Pathway: Autoinducer AI-2 Biosynthesis
• Reaction:
  1. S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L- homocysteine + adenine
  2. 5'-deoxyadenosine + H2O = 5-deoxy-D-ribose + adenine
  3. S-methyl-5'-thioadenosine + H2O = 5-(methylsulfanyl)-D-ribose + adenine

S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L- homocysteine + adenine (Bound ligand (Het Group name = ADE) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site