PDBsum entry 4jrt

RNA

PDB id: 4jrt

Contents

DNA/RNA

Waters ×23

PDB id:

4jrt

Name:

RNA

Title:

Crystal structure of an a-form RNA duplex containing three gu base pairs

Structure:

RNA (5'-r(p Gp Gp Gp Up Gp Gp Up Gp Cp Gp Gp G)-3'). Chain: a. Engineered: yes. RNA (5'-r(p Cp Cp Up Gp Cp Ap Cp Up Gp Cp Cp C)-3'). Chain: b. Engineered: yes

Source:

Synthetic: yes. Other_details: in vitro transcription. Other_details: in vitro transcription

Resolution:

2.60Å R-factor: 0.185 R-free: 0.228

Authors:

J.Kondo,A.C.Dock-Bregeon,D.K.Willkomm,R.K.Hartmann,E.Westhof

Key ref:

J.Kondo et al. (2013). Structure of an A-form RNA duplex obtained by degradation of 6S RNA in a crystallization droplet. Acta Crystallogr Sect F Struct Biol Cryst Commun, 69, 634-639. PubMed id: 23722840 DOI: 10.1107/S1744309113013018

Date:

21-Mar-13 Release date: 05-Jun-13

DNA/RNA chains

G-G-G-U-G-G-U-G-C-G-G-G 12 bases

C-C-U-G-C-A-C-U-G-C-C-C 12 bases

DOI no: 10.1107/S1744309113013018

Acta Crystallogr Sect F Struct Biol Cryst Commun 69:634-639 (2013)

PubMed id: 23722840

Structure of an A-form RNA duplex obtained by degradation of 6S RNA in a crystallization droplet.

J.Kondo, A.C.Dock-Bregeon, D.K.Willkomm, R.K.Hartmann, E.Westhof.

ABSTRACT

In the course of a crystallographic study of a 132 nt variant of Aquifex aeolicus 6S RNA, a crystal structure of an A-form RNA duplex containing 12 base pairs was solved at a resolution of 2.6 Å. In fact, the RNA duplex is part of the 6S RNA and was obtained by accidental but precise degradation of the 6S RNA in a crystallization droplet. 6S RNA degradation was confirmed by microscopic observation of crystals and gel electrophoresis of crystallization droplets. The RNA oligomers obtained form regular A-form duplexes containing three GoU wobble-type base pairs, one of which engages in intermolecular contacts through a ribose-zipper motif at the crystal-packing interface.