PDBsum entry 4jrf

Cell adhesion

PDB id: 4jrf

Contents

Protein chain

479 a.a.

Ligands

_CL ×4

SO4 ×8

PG4 ×4

Metals

_CA

Waters ×357

PDB id:

4jrf

Name:

Cell adhesion

Title:

Crystal structure of a putative cell adhesion protein (bacova_01548) from bacteroides ovatus atcc 8483 at 1.98 a resolution (psi community target, nakayama)

Structure:

Putative cell adhesion protein. Chain: a. Engineered: yes

Source:

Bacteroides ovatus. Organism_taxid: 411476. Strain: atcc 8483. Gene: zp_02064579.1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.98Å R-factor: 0.157 R-free: 0.178

Authors:

Joint Center For Structural Genomics (Jcsg)

Key ref:

Q.Xu et al. (2016). A Distinct Type of Pilus from the Human Microbiome. Cell, 165, 690-703. PubMed id: 27062925

Date:

21-Mar-13 Release date: 03-Apr-13

Protein chain

A7LUP9  (A7LUP9_BACOV) -

Cell 165:690-703 (2016)

PubMed id: 27062925

A Distinct Type of Pilus from the Human Microbiome.

Q.Xu, M.Shoji, S.Shibata, M.Naito, K.Sato, M.A.Elsliger, J.C.Grant, H.L.Axelrod, H.J.Chiu, C.L.Farr, L.Jaroszewski, M.W.Knuth, A.M.Deacon, A.Godzik, S.A.Lesley, M.A.Curtis, K.Nakayama, I.A.Wilson.

ABSTRACT

Pili are proteinaceous polymers of linked pilins that protrude from the cell surface of many bacteria and often mediate adherence and virulence. We investigated a set of 20 Bacteroidia pilins from the human microbiome whose structures and mechanism of assembly were unknown. Crystal structures and biochemical data revealed a diverse protein superfamily with a common Greek-key β sandwich fold with two transthyretin-like repeats that polymerize into a pilus through a strand-exchange mechanism. The assembly mechanism of the central, structural pilins involves proteinase-assisted removal of their N-terminal β strand, creating an extended hydrophobic groove that binds the C-terminal donor strands of the incoming pilin. Accessory pilins at the tip and base have unique structural features specific to their location, allowing initiation or termination of the assembly. The Bacteroidia pilus, therefore, has a biogenesis mechanism that is distinct from other known pili and likely represents a different type of bacterial pilus.