PDBsum entry 4jqw

Apoptosis/signalling protein

PDB id: 4jqw

Contents

Protein chains

92 a.a.

73 a.a.

Ligands

PO4

PDB id:

4jqw

Name:

Apoptosis/signalling protein

Title:

Crystal structure of a complex of nod1 card and ubiquitin

Structure:

Nucleotide-binding oligomerization domain-containing protein 1. Chain: a. Fragment: card domain. Synonym: caspase recruitment domain-containing protein 4. Engineered: yes. Polyubiquitin-c. Chain: c. Fragment: ubiquitin.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: card4, nod1. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: ubc.

Resolution:

2.90Å R-factor: 0.230 R-free: 0.270

Authors:

A.M.Ver Heul,L.Gakhar,R.C.Piper,S.Ramaswamy

Key ref:

A.M.Ver Heul et al. (2014). Crystal structure of a complex of NOD1 CARD and ubiquitin. Plos One, 9, e104017. PubMed id: 25127239 DOI: 10.1371/journal.pone.0104017

Date:

20-Mar-13 Release date: 26-Mar-14

Protein chain

Q9Y239  (NOD1_HUMAN) -  Nucleotide-binding oligomerization domain-containing protein 1 from Homo sapiens

Seq: 953 a.a.

Struc: 92 a.a.*

Protein chain

P0CG48  (UBC_HUMAN) -  Polyubiquitin-C from Homo sapiens

Seq: 685 a.a.

Struc: 73 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1371/journal.pone.0104017

Plos One 9:e104017 (2014)

PubMed id: 25127239

Crystal structure of a complex of NOD1 CARD and ubiquitin.

A.M.Ver Heul, L.Gakhar, R.C.Piper, R.Subramanian.

ABSTRACT

The Caspase Recruitment Domain (CARD) from the innate immune receptor NOD1 was crystallized with Ubiquitin (Ub). NOD1 CARD was present as a helix-swapped homodimer similar to other structures of NOD1 CARD, and Ub monomers formed a homodimer similar in conformation to Lys48-linked di-Ub. The interaction between NOD1 CARD and Ub in the crystal was mediated by novel binding sites on each molecule. Comparisons of these sites to previously identified interaction surfaces on both molecules were made along with discussion of their potential functional significance.