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PDBsum entry 4jk1
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Transcription, transferase
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PDB id
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4jk1
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Contents |
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323 a.a.
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221 a.a.
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1335 a.a.
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1160 a.a.
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90 a.a.
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517 a.a.
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76 a.a.
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458 a.a.
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PDB id:
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| Name: |
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Transcription, transferase
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Title:
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X-ray crystal structure of escherichia coli sigma70 holoenzyme in complex with guanosine tetraphosphate (ppgpp)
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Structure:
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Escherichia coli RNA polymerase alpha subunit. Chain: a, b, f, g. Synonym: rnap subunit alpha, RNA polymerase subunit alpha, transcriptase subunit alpha. Engineered: yes. Escherichia coli RNA polymerase beta subunit. Chain: c, h. Synonym: rnap subunit beta, RNA polymerase subunit beta, transcriptase subunit beta.
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Source:
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Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: rpoa, pez, phs, sez, b3295, jw3257. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: rpob, gron, nitb, rif, ron, stl, stv, tabd, b3987, jw3950. Gene: rpoc, tabb, b3988, jw3951. Organism_taxid: 1110693.
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Resolution:
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3.90Å
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R-factor:
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0.255
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R-free:
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0.320
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Authors:
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K.S.Murakami
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Key ref:
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U.Mechold
et al.
(2013).
Differential regulation by ppGpp versus pppGpp in Escherichia coli.
Nucleic Acids Res,
41,
6175-6189.
PubMed id:
DOI:
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Date:
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09-Mar-13
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Release date:
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10-Apr-13
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PROCHECK
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Headers
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References
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P0A7Z4
(RPOA_ECOLI) -
DNA-directed RNA polymerase subunit alpha from Escherichia coli (strain K12)
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Seq:
Struc:
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329 a.a.
323 a.a.
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P0A7Z4
(RPOA_ECOLI) -
DNA-directed RNA polymerase subunit alpha from Escherichia coli (strain K12)
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Seq:
Struc:
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329 a.a.
221 a.a.
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P0A8V2
(RPOB_ECOLI) -
DNA-directed RNA polymerase subunit beta from Escherichia coli (strain K12)
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Seq:
Struc:
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1342 a.a.
1335 a.a.
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P0A8T7
(RPOC_ECOLI) -
DNA-directed RNA polymerase subunit beta' from Escherichia coli (strain K12)
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Seq:
Struc:
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1407 a.a.
1160 a.a.
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P0A800
(RPOZ_ECOLI) -
DNA-directed RNA polymerase subunit omega from Escherichia coli (strain K12)
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Seq:
Struc:
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91 a.a.
90 a.a.
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P00579
(RPOD_ECOLI) -
RNA polymerase sigma factor RpoD from Escherichia coli (strain K12)
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Seq:
Struc:
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613 a.a.
517 a.a.
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Enzyme class 2:
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Chains A, B, C, D, E, F, G, H, I, J:
E.C.2.7.7.6
- DNA-directed Rna polymerase.
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Reaction:
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RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
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RNA(n)
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+
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ribonucleoside 5'-triphosphate
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=
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RNA(n+1)
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+
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diphosphate
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Enzyme class 3:
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Chains X, Y:
E.C.?
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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DOI no:
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Nucleic Acids Res
41:6175-6189
(2013)
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PubMed id:
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Differential regulation by ppGpp versus pppGpp in Escherichia coli.
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U.Mechold,
K.Potrykus,
H.Murphy,
K.S.Murakami,
M.Cashel.
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ABSTRACT
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