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PDBsum entry 4jb9
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Viral protein/immune system
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PDB id
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4jb9
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Contents |
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344 a.a.
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225 a.a.
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209 a.a.
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PDB id:
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| Name: |
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Viral protein/immune system
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Title:
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Crystal structure of antibody vrc06 in complex with HIV-1 gp120 core
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Structure:
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Clade a/e 93th057 HIV-1 gp120 core. Chain: g. Engineered: yes. Antibody vrc06 heavy chain. Chain: h. Engineered: yes. Antibody vrc06 light chain. Chain: l. Engineered: yes
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Source:
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Human immunodeficiency virus 1. Organism_taxid: 11676. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: 293f. Homo sapiens. Human. Organism_taxid: 9606.
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Resolution:
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2.60Å
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R-factor:
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0.250
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R-free:
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0.287
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Authors:
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Y.D.Kwon,T.Zhou,S.Srivatsan,P.D.Kwong
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Key ref:
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I.S.Georgiev
et al.
(2013).
Delineating antibody recognition in polyclonal sera from patterns of HIV-1 isolate neutralization.
Science,
340,
751-756.
PubMed id:
DOI:
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Date:
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19-Feb-13
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Release date:
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08-May-13
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PROCHECK
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Headers
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References
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Q0ED31
(Q0ED31_HV1) -
Envelope glycoprotein gp160 from Human immunodeficiency virus type 1
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Seq:
Struc:
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857 a.a.
344 a.a.*
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DOI no:
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Science
340:751-756
(2013)
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PubMed id:
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Delineating antibody recognition in polyclonal sera from patterns of HIV-1 isolate neutralization.
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I.S.Georgiev,
N.A.Doria-Rose,
T.Zhou,
Y.D.Kwon,
R.P.Staupe,
S.Moquin,
G.Y.Chuang,
M.K.Louder,
S.D.Schmidt,
H.R.Altae-Tran,
R.T.Bailer,
K.McKee,
M.Nason,
S.O'Dell,
G.Ofek,
M.Pancera,
S.Srivatsan,
L.Shapiro,
M.Connors,
S.A.Migueles,
L.Morris,
Y.Nishimura,
M.A.Martin,
J.R.Mascola,
P.D.Kwong.
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ABSTRACT
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Serum characterization and antibody isolation are transforming our understanding
of the humoral immune response to viral infection. Here, we show that epitope
specificities of HIV-1-neutralizing antibodies in serum can be elucidated from
the serum pattern of neutralization against a diverse panel of HIV-1 isolates.
We determined "neutralization fingerprints" for 30 neutralizing
antibodies on a panel of 34 diverse HIV-1 strains and showed that similarity in
neutralization fingerprint correlated with similarity in epitope. We used these
fingerprints to delineate specificities of polyclonal sera from 24
HIV-1-infected donors and a chimeric siman-human immunodeficiency virus-infected
macaque. Delineated specificities matched published specificities and were
further confirmed by antibody isolation for two sera. Patterns of virus-isolate
neutralization can thus afford a detailed epitope-specific understanding of
neutralizing-antibody responses to viral infection.
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Links
