PDBsum entry 4jb1

Oxidoreductase

PDB id: 4jb1

Contents

Protein chain

337 a.a.

Ligands

FAD

NAP

GOL ×6

Waters ×164

PDB id:

4jb1

Name:

Oxidoreductase

Title:

Crystal structure of p. Aeruginosa murb in complex with NADP+

Structure:

Udp-n-acetylenolpyruvoylglucosamine reductase. Chain: a. Synonym: udp-n-acetylmuramate dehydrogenase. Engineered: yes

Source:

Pseudomonas aeruginosa. Organism_taxid: 208964. Strain: pao1. Gene: murb, pa2977. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.10Å R-factor: 0.183 R-free: 0.236

Authors:

M.W.Chen,B.Lohkamp,R.Schnell,J.Lescar,G.Schneider

Key ref:

M.W.Chen et al. (2013). Substrate Channel Flexibility in Pseudomonas aeruginosa MurB Accommodates Two Distinct Substrates. Plos One, 8, e66936. PubMed id: 23805286 DOI: 10.1371/journal.pone.0066936

Date:

19-Feb-13 Release date: 17-Jul-13

Protein chain

Q9HZM7  (MURB_PSEAE) -  UDP-N-acetylenolpyruvoylglucosamine reductase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Seq: 339 a.a.

Struc: 337 a.a.

Enzyme reactions

• Enzyme class: E.C.1.3.1.98 - UDP-N-acetylmuramate dehydrogenase.
• Reaction: UDP-N-acetyl-alpha-D-muramate + NADP⁺ = UDP-N-acetyl-3- O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH + H⁺

UDP-N-acetyl-alpha-D-muramate (Bound ligand (Het Group name = NAP) corresponds exactly) + NADP(+) = UDP-N-acetyl-3- O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH + H(+)

• Cofactor: FAD

FAD (Bound ligand (Het Group name = FAD) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1371/journal.pone.0066936

Plos One 8:e66936 (2013)

PubMed id: 23805286

Substrate Channel Flexibility in Pseudomonas aeruginosa MurB Accommodates Two Distinct Substrates.

M.W.Chen, B.Lohkamp, R.Schnell, J.Lescar, G.Schneider.

ABSTRACT

No abstract given.