PDBsum entry 4j9d

Transferase/unknown function

PDB id: 4j9d

Contents

Protein chains

56 a.a.

11 a.a.

59 a.a.

Ligands

SO4

Waters ×200

PDB id:

4j9d

Name:

Transferase/unknown function

Title:

Crystal structure of the n114a mutant of the abl-sh3 domain complexed with the high affinity peptide p0

Structure:

Tyrosine-protein kinase abl1. Chain: a, c, e. Fragment: sh3 domain (unp residues 60-121). Synonym: abelson murine leukemia viral oncogene homolog 1, abelson tyrosine-protein kinase 1, proto-oncogenE C-abl, p150. Engineered: yes. Mutation: yes. P0. Chain: b, d, f.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: abl, abl1, jtk7. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: this sequence has been optimized to increase the affinity

Resolution:

1.50Å R-factor: 0.145 R-free: 0.183

Authors:

A.Camara-Artigas

Key ref:

A.Camara-Artigas Crystal structure of the n114a mutant of the abl-Sh3 complexed with the high affinity peptide p0. To be published, .

Date:

16-Feb-13 Release date: 29-Jan-14

Protein chains

P00519  (ABL1_HUMAN) -  Tyrosine-protein kinase ABL1 from Homo sapiens

Seq: 1130 a.a.

Struc: 56 a.a.*

Protein chains

Q9Y3L3  (3BP1_HUMAN) -  SH3 domain-binding protein 1 from Homo sapiens

Seq: 701 a.a.

Struc: 10 a.a.*

Protein chain

P00519  (ABL1_HUMAN) -  Tyrosine-protein kinase ABL1 from Homo sapiens

Seq: 1130 a.a.

Struc: 59 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, C, E: E.C.2.7.10.2 - non-specific protein-tyrosine kinase.
• Reaction: L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site